Product Number |
ARP52288_P050 |
Product Page |
www.avivasysbio.com/nup50-antibody-c-terminal-region-arp52288-p050.html |
Name |
NUP50 Antibody - C-terminal region (ARP52288_P050) |
Protein Size (# AA) |
468 amino acids |
Molecular Weight |
50kDa |
NCBI Gene Id |
10762 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Nucleoporin 50kDa |
Alias Symbols |
NPAP60, NPAP60L |
Peptide Sequence |
Synthetic peptide located within the following region: TTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEED |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Olsen,J.V., (2006) Cell 127 (3), 635-648 |
Description of Target |
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. |
Protein Interactions |
KPNA5; KPNA4; KPNA3; KPNA2; KPNA1; KPNA6; UBC; KIAA1033; SART3; TTI1; TSC22D1; IKBKAP; KIF5B; EPB41L1; ACACA; SRPK2; LMNA; HDAC6; NPM1; UBAP2; TOR1AIP1; NUP153; APP; CUL3; Mki67; Kifc5b; SLX4; KPNB1; XPO1; RAN; CDKN1B; IPO13; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-NUP50 (ARP52288_P050) antibody |
Blocking Peptide |
For anti-NUP50 (ARP52288_P050) antibody is Catalog # AAP52288 (Previous Catalog # AAPS30802) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human NUP50 |
Uniprot ID |
Q9UKX7 |
Protein Name |
Nuclear pore complex protein Nup50 |
Sample Type Confirmation |
There is BioGPS gene expression data showing that NUP50 is expressed in HepG2 NUP50 is supported by BioGPS gene expression data to be expressed in HeLa |
Protein Accession # |
NP_009103 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_007172 |
Tested Species Reactivity |
Human |
Gene Symbol |
NUP50 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Yeast |
Application |
IF, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 85%; Horse: 100%; Human: 100%; Mouse: 91%; Pig: 100%; Rabbit: 100%; Rat: 92%; Yeast: 80% |
Image 1 | Human HepG2
| WB Suggested Anti-NUP50 Antibody Titration: 0.2-1 ug/ml Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that NUP50 is expressed in HepG2 |
|
Image 2 | Human HepG2
| Host: Rabbit Target Name: NUP50 Sample Type: HepG2 Antibody Dilution: 1.0ug/mlThere is BioGPS gene expression data showing that NUP50 is expressed in HepG2 |
|
Image 3 | Human Hela
| Host: Rabbit Target Name: NUP50 Sample Type: Hela Antibody Dilution: 1.0ug/mlNUP50 is supported by BioGPS gene expression data to be expressed in HeLa |
|
Image 4 | Human HeLa
| Sample Type : Hela cell lysate
|
|