CTTN Antibody - N-terminal region (ARP52067_P050)

• Product Number: ARP52067_P050
• Name: CTTN Antibody - N-terminal region (ARP52067_P050)
• Protein Size (# AA): 550 amino acids
• Molecular Weight: 61kDa
• NCBI Gene Id: 2017
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Cortactin
• Alias Symbols: EMS1
• Peptide Sequence: Synthetic peptide located within the following region: KHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ma,S.F., (er) Genet. Epidemiol. (2008) In press
• Description of Target: CTTN is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. CTTN is localized in the cytoplasm and in areas of the cell-substratum contacts. It has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, CTTN is degraded in a caspase-dependent manner. The aberrant regulation of t CTTN contributes to tumor cell invasion and metastasis.This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
• Protein Interactions: SPRR2A; CDC37; CTTN; UBC; NEDD8; RPA3; RPA2; RPA1; VTA1; GSPT2; ATG7; UGDH; ME1; IDE; GSPT1; GARS; CTNNA1; ATP6V1C1; APEH; STAT1; SSB; SNX2; SNX1; SHMT2; SHMT1; SARS; RDX; ACTR2; NPM1; LMNA; ACTG1; ACTR3; PAN2; ATF2; DNM2; NCF1; Mbp; ASAP1; CORO1B; SDHAF2
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-CTTN (ARP52067_P050) antibody is Catalog # AAP52067 (Previous Catalog # AAPP30292)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CTTN
• Uniprot ID: Q8N707
• Protein Name: Src substrate cortactin
• Protein Accession #: NP_005222
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005231
• Tested Species Reactivity: Human
• Gene Symbol: CTTN
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human Brain
  
  WB Suggested Anti-CTTN Antibody Titration: 0.2-1 ug/ml
  Positive Control: Human brain
• Image 2: Human Bronchial Epithelial Tissue
  
  CTTN antibody - N-terminal region (ARP52067_P050)
  Catalog Number: ARP52067_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
  Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 3: Human Fetal Lung
  
  Host: Rabbit
  Target Name: CTTN
  Sample Type: Human Fetal Lung
  Antibody Dilution: 1.0ug/ml
• Image 4: Human Fetal Liver
  
  Host: Rabbit
  Target Name: CTTN
  Sample Type: Human Fetal Liver
  Antibody Dilution: 1.0ug/ml
• Image 5: Human Fetal Muscle
  
  Host: Rabbit
  Target Name: CTTN
  Sample Type: Human Fetal Muscle
  Antibody Dilution: 1.0ug/ml