COPA Antibody - N-terminal region (ARP51893_P050)

• Product Number: ARP51893_P050
• Name: COPA Antibody - N-terminal region (ARP51893_P050)
• Protein Size (# AA): 1224 amino acids
• Molecular Weight: 138 kDa
• Subunit: alpha
• NCBI Gene Id: 1314
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Coatomer protein complex, subunit alpha
• Alias Symbols: AILJK, HEP-COP, alpha-COP
• Peptide Sequence: Synthetic peptide located within the following region: PWILTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGGD
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Olsen,J.V., (2006) Cell 127 (3), 635-648
• Description of Target: In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone.In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms.
• Protein Interactions: UBC; SUMO2; SMN1; STAU1; NUDCD1; SUMO1; NEDD8; RPA3; RPA2; RPA1; SUZ12; RNF2; COPZ1; DHX38; COPB2; CCT3; TCP1; SEPT2; ARCN1; UBD; VCAM1; ITGA4; COPB1; COPG2; COPG1; COPE; SEPT9; SEC23A; SEC24C; UBXN6; FBXO6; LMAN1; SACM1L; DDX20; TMED10; GEMIN2; COPA; CDK
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-COPA (ARP51893_P050) antibody is Catalog # AAP51893 (Previous Catalog # AAPP40072)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human COPA
• Uniprot ID: P53621
• Protein Name: Coatomer subunit alpha
• Publications:

  a-COP binding to the survival motor neuron protein SMN is required for neuronal process outgrowth. Hum. Mol. Genet. 24, 7295-307 (2015). 26464491

  Ting, C.-H. et al. The spinal muscular atrophy disease protein SMN is linked to the Golgi network. PLoS One 7, e51826 (2012). 23284781

• Sample Type Confirmation:

  COPA is strongly supported by BioGPS gene expression data to be expressed in HeLa

• Protein Accession #: NP_004362
• Purification: Affinity Purified
• Nucleotide Accession #: NM_004371
• Tested Species Reactivity: Human
• Gene Symbol: COPA
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 86%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 93%; Zebrafish: 100%
• Image 1: Hela, Human heart
  
  Host: Rabbit
  Target: COPA
  Positive control (+): Hela (HL)
  Negative control (-): Human heart (HE)
  Antibody concentration: 1ug/ml