FZR1 Antibody - N-terminal region (ARP51279_P050)

• Product Number: ARP51279_P050
• Name: FZR1 Antibody - N-terminal region (ARP51279_P050)
• Protein Size (# AA): 493 amino acids
• Molecular Weight: 55kDa
• NCBI Gene Id: 51343
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Fizzy/cell division cycle 20 related 1 (Drosophila)
• Alias Symbols: FZR, CDH1, FZR2, HCDH, HCDH1, CDC20C
• Peptide Sequence: Synthetic peptide located within the following region: SSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKSPSQNRKAKDATSDN
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. FZR1 associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
• Protein Interactions: ZC3HC1; CDC25A; CCNB1; UBE2S; UBE2C; PTTG1; UBE2D2; TP53; CDR2; UBE2K; ANAPC4; PDS1; STAU1; CDK2; CDC27; RACGAP1; FOXM1; HECW2; PHF8; BTRC; CCNA1; UBC; SOX2; SKP2; PLK1; CUL1; CUEDC2; CDC20; kif22; SIRT2; CLSPN; ANAPC11; CDC14B; VHL; PTEN; EYA1; DAXX; BUB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: Catalog # AAP51279 (Previous Catalog # AAPS22306)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FZR1
• Uniprot ID: Q9UM11
• Protein Name: Fizzy-related protein homolog
• Publications:

  Downregulation of Cdh1 signalling in spinal dorsal horn contributes to the maintenance of mechanical allodynia after nerve injury in rats. Mol Pain. 12, (2016). 27184142

• Sample Type Confirmation:

  FZR1 is strongly supported by BioGPS gene expression data to be expressed in 721_B

• Protein Accession #: NP_057347
• Purification: Affinity Purified
• Nucleotide Accession #: NM_016263
• Tested Species Reactivity: Human
• Gene Symbol: FZR1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human Adult Placenta
  
  Host: Rabbit
  Target Name: FZR1
  Sample Type: Human Adult Placenta
  Antibody Dilution: 1.0ug/ml
• Image 2: Human Fetal Lung
  
  Host: Rabbit
  Target Name: FZR1
  Sample Type: Human Fetal Lung
  Antibody Dilution: 1.0ug/ml
• Image 3: Human Fetal Heart
  
  Host: Rabbit
  Target Name: FZR1
  Sample Type: Human Fetal Heart
  Antibody Dilution: 1.0ug/ml
• Image 4: Human Fetal Brain
  
  Host: Rabbit
  Target Name: FZR1
  Sample Type: Human Fetal Brain
  Antibody Dilution: 1.0ug/ml
• Image 5: Human 721_B
  
  WB Suggested Anti-FZR1 Antibody Titration: 0.2-1 ug/ml
  Positive Control: 721_B cell lysateFZR1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
• Image 6: Human A549 Whole Cell
  
  Host: Rabbit
  Target Name: FZR1
  Sample Tissue: Human A549 Whole Cell
  Antibody Dilution: 1ug/ml