SRPRB Antibody - middle region (ARP49643_P050)

• Product Number: ARP49643_P050
• Name: SRPRB Antibody - middle region (ARP49643_P050)
• Protein Size (# AA): 271 amino acids
• Molecular Weight: 30 kDa
• Subunit: beta
• NCBI Gene Id: 58477
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Signal recognition particle receptor, B subunit
• Alias Symbols: APMCF1, SR-beta
• Peptide Sequence: Synthetic peptide located within the following region: QDIAMAKSAKLIQQQLEKELNTLRVTRSAAPSTLYSSSTAPAQLGKKGKE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)
• Description of Target: SRPRB has similarity to mouse protein which is a subunit of the signal recognition particle receptor (SR). This subunit is a transmembrane GTPase belonging to the GTPase superfamily. It anchors alpha subunit, a peripheral membrane GTPase, to the ER membrane. SR is required for the cotranslational targeting of both secretory and membrane proteins to the ER membrane.The protein encoded by this gene has similarity to mouse protein which is a subunit of the signal recognition particle receptor (SR). This subunit is a transmembrane GTPase belonging to the GTPase superfamily. It anchors alpha subunit, a peripheral membrane GTPase, to the ER membrane. SR is required for the cotranslational targeting of both secretory and membrane proteins to the ER membrane.The protein encoded by this gene has similarity to mouse protein which is a subunit of the signal recognition particle receptor (SR). This subunit is a transmembrane GTPase belonging to the GTPase superfamily. It anchors alpha subunit, a peripheral membrane GTPase, to the ER membrane. SR is required for the cotranslational targeting of both secretory and membrane proteins to the ER membrane.
• Protein Interactions: ERLIN2; UBC; STAU1; MDM2; FBXO6; PPP6R1; ADRB2; LMNA; UBL4A; CA9; UBE2L3; TAGLN; SPR; SNRPC; S100A10; RRBP1; RPL38; RFX5; RBMS1; PIN4; PFN1; ACAA1; TMED4; TMEM65; RPS10P5; MRRF; SARNP; NABP2; MRPL14; TMBIM1; SUGP1; SBDS; MRPS28; TARDBP; TOMM40; UBE4B; SF3
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-SRPRB (ARP49643_P050) antibody is Catalog # AAP49643 (Previous Catalog # AAPY02721)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SRPRB
• Uniprot ID: Q9Y5M8
• Protein Name: Signal recognition particle receptor subunit beta
• Sample Type Confirmation:

  SRPRB is supported by BioGPS gene expression data to be expressed in 721_B, HeLa, Jurkat

• Protein Accession #: NP_067026
• Purification: Affinity Purified
• Nucleotide Accession #: NM_021203
• Tested Species Reactivity: Human
• Gene Symbol: SRPRB
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Yeast, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 93%
• Image 1: Human Hela
  
  Host: Rabbit
  Target Name: SRPRB
  Sample Type: Hela
  Antibody Dilution: 1.0ug/mlSRPRB is supported by BioGPS gene expression data to be expressed in HeLa
• Image 2: Human 721_B
  
  Host: Rabbit
  Target Name: SRPRB
  Sample Type: 721_B
  Antibody Dilution: 1.0ug/mlSRPRB is supported by BioGPS gene expression data to be expressed in 721_B
• Image 3: Human Bronchial Epithelial Tissue
  
  Rabbit Anti-SRPRB Antibody
  Catalog Number: ARP49643_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
  Observed Staining: Cytoplasmic
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1/600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 4:
  
  25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.