VISA Antibody - N-terminal region (ARP49558_P050)

• Product Number: ARP49558_P050
• Name: VISA Antibody - N-terminal region (ARP49558_P050)
• Protein Size (# AA): 896 amino acids
• Molecular Weight: 56kDa
• NCBI Gene Id: 57506
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Mitochondrial antiviral signaling protein
• Alias Symbols: IPS1, VISA, IPS-1, CARDIF
• Peptide Sequence: Synthetic peptide located within the following region: ETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGH
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Lad,S.P., (2008) Mol. Immunol. 45 (8), 2277-2287
• Description of Target: Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 or by the cytoplasmic RNA helicases MDA5 and RIGI (ROBO3). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK) and IKKB (IKBKB), which activate NFKB, or TBK1 and IKKE (IKBKE), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1). For RIGI, the intermediary protein is mitochondria-bound VISA. Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: MIB2; KCNIP3; DDX58; UBC; TRAF6; TRAF3; UBXN1; TMEM173; SMURF2; HCVgp1; AMFR; MFN1; MFN2; MUL1; TOMM70A; FLOT2; NDFIP1; SMURF1; TBK1; IKBKG; TICAM1; TRAF2; MAP3K7; SRC; IRF7; IRF3; IKBKE; TSPAN6; RARRES3; ECSIT; PCBP2; ITCH; IFIH1; PSMA7; WDR5; HECTD3; PD
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-MAVS (ARP49558_P050) antibody is Catalog # AAP49558 (Previous Catalog # AAPS22409)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human VISA
• Uniprot ID: Q2HWT5
• Protein Name: Mitochondrial antiviral-signaling protein
• Sample Type Confirmation:

  MAVS is supported by BioGPS gene expression data to be expressed in HEK293T

• Protein Accession #: NP_065797
• Purification: Affinity Purified
• Nucleotide Accession #: NM_020746
• Tested Species Reactivity: Human
• Gene Symbol: MAVS
• Predicted Species Reactivity: Human, Mouse, Cow
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 79%; Human: 100%; Mouse: 79%
• Image 1: Transfected 293T
  
  WB Suggested Anti-VISA Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:12500
  Positive Control: Transfected 293T
• Image 2: Human 293T
  
  Researcher: Dr. Safia Deddouche, London Research Institute
  Application: IHC
  Species+tissue/cell type:293T cells transfected with 3FLAG-VISA
  Primary antibody dilution: 1:500
  Secondary antibody: Anti-rabbit-Alexa 488
  Secondary antibody dilution:1:500
• Image 3: Human HEK293T
  
  Sample Type:
  1: 20ug HEK293T no transfection, 2: 20ug HEK293T 3Flag-MAVS/VISA
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit HRP
  Secondary Antibody Dilution:
  1:1000
  Color/Signal Descriptions:
  ARP49558-QC17479-WB-image-02
  Gene Name:
  VISA
  Submitted by:
  Dr. Safia Deddouche Immunobiology Laboratory Cancer Research UK London Research Institute Lincoln's Inn Fields Laboratories 44 Lincoln's Inn Fields London WC2A 3LY UKMAVS is supported by BioGPS gene expression data to be expressed in HEK293T