MAP2K2 Antibody - C-terminal region (ARP48942_P050)

• Product Number: ARP48942_P050
• Name: MAP2K2 Antibody - C-terminal region (ARP48942_P050)
• Protein Size (# AA): 400 amino acids
• Molecular Weight: 44kDa
• NCBI Gene Id: 5605
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Mitogen-activated protein kinase kinase 2
• Alias Symbols: CFC4, MEK2, MKK2, MAPKK2, PRKMK2
• Peptide Sequence: Synthetic peptide located within the following region: IKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQPGTPTRTAV
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Adjei,A.A., (2008) J. Clin. Oncol. 26 (13), 2139-2146
• Description of Target: MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in MAP2K2 gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: CCNDBP1; RAF1; SUMO2; UBC; EGFR; IQGAP1; NFE2L2; YWHAB; MAPK1; PEX14; BRAF; ARAF; FAM65B; ZNF207; LIG4; MAPK8; WDR83; Ksr1; COPS5; MAP2K1; MEPCE; GRIN1; CNKSR1; LAMTOR3; GRIN2D; RGS12; CASP9; MAPK3; MAP2K2; DUSP3; Flna;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-MAP2K2 (ARP48942_P050) antibody is Catalog # AAP48942 (Previous Catalog # AAPP28989)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MAP2K2
• Uniprot ID: P36507
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that MAP2K2 is expressed in HepG2

• Protein Accession #: NP_109587
• Purification: Affinity Purified
• Nucleotide Accession #: NM_030662
• Tested Species Reactivity: Human
• Gene Symbol: MAP2K2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 92%; Horse: 93%; Human: 100%; Mouse: 85%; Pig: 93%; Rat: 92%
• Image 1: Human Pancreas
  
  Human Pancreas
• Image 2: Human HepG2
  
  WB Suggested Anti-MAP2K2 Antibody Titration: 0.2-1 ug/ml
  Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that MAP2K2 is expressed in HepG2
• Image 3: Human Lung Tissue
  
  MAP2K2 antibody - C-terminal region (ARP48942_P050)
  Catalog Number: ARP48942_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
  Observed Staining: Cytoplasm of pneumocytes
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec