VIM Antibody - N-terminal region (ARP48225_P050)

Data Sheet
 
Product Number ARP48225_P050
Product Page www.avivasysbio.com/vim-antibody-n-terminal-region-arp48225-p050.html
Name VIM Antibody - N-terminal region (ARP48225_P050)
Protein Size (# AA) 466 amino acids
Molecular Weight 54kDa
NCBI Gene Id 7431
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Vimentin
Alias Symbols FLJ36605
Peptide Sequence Synthetic peptide located within the following region: LNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRR
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Dawson,S.J. (2008) Biochemistry 47 (18), 5127-5138
Description of Target Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Interactions PKN1; PPL; PLA2G2A; PA2G4; KRT20; TXLNB; CWF19L2; PNMA5; UBC; TP53; CHFR; SPRTN; SUMO2; SUMO3; TRIM68; STAU1; ASB15; ASB9; ASB16; ASB2; RNF2; EEF1D; SERPINH1; COPS8; FOXK1; YWHAQ; PARK2; FBXO6; CASP7; CASP3; KAT7; UBD; PINK1; BAG3; GSK3B; PAN2; CAPN1; SVI
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-VIM (ARP48225_P050) antibody
Blocking Peptide For anti-VIM (ARP48225_P050) antibody is Catalog # AAP48225 (Previous Catalog # AAPS22911)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human VIM
Uniprot ID P08670
Protein Name Vimentin
Protein Accession # NP_003371
Purification Affinity Purified
Nucleotide Accession # NM_003380
Tested Species Reactivity Human, Mouse
Gene Symbol VIM
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%
Image 1
Human brain
WB Suggested Anti-VIM Antibody Titration: 0.2-1 ug/ml
Positive Control: Human brain
Image 2
Mouse Small Intestine
Host: Mouse
Target Name: VIM
Sample Tissue: Mouse Small Intestine
Antibody Dilution: 1ug/ml
Image 3
Human Ovary
Rabbit Anti-VIM Antibody
Catalog Number: ARP48225_P050
Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 4
Human COS7
submitted by:Carl LundinStanford UniversityThe Vimentin antibody ARP48225_P050 works well for visualization of the vimentin cytoskeleton (see attached images). We used PFA-fixed COS-7 cells and an antibody dilution of 1:400.
Image 5
Human brain stem
Sample Type:
Human brain stem cells
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
VIM: Red DAPI:Blue
Gene Name:
VIM
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com