Product Number |
ARP48225_P050 |
Product Page |
www.avivasysbio.com/vim-antibody-n-terminal-region-arp48225-p050.html |
Name |
VIM Antibody - N-terminal region (ARP48225_P050) |
Protein Size (# AA) |
466 amino acids |
Molecular Weight |
54kDa |
NCBI Gene Id |
7431 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Vimentin |
Alias Symbols |
FLJ36605 |
Peptide Sequence |
Synthetic peptide located within the following region: LNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRR |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Dawson,S.J. (2008) Biochemistry 47 (18), 5127-5138 |
Description of Target |
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Protein Interactions |
PKN1; PPL; PLA2G2A; PA2G4; KRT20; TXLNB; CWF19L2; PNMA5; UBC; TP53; CHFR; SPRTN; SUMO2; SUMO3; TRIM68; STAU1; ASB15; ASB9; ASB16; ASB2; RNF2; EEF1D; SERPINH1; COPS8; FOXK1; YWHAQ; PARK2; FBXO6; CASP7; CASP3; KAT7; UBD; PINK1; BAG3; GSK3B; PAN2; CAPN1; SVI |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-VIM (ARP48225_P050) antibody |
Blocking Peptide |
For anti-VIM (ARP48225_P050) antibody is Catalog # AAP48225 (Previous Catalog # AAPS22911) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human VIM |
Uniprot ID |
P08670 |
Protein Name |
Vimentin |
Protein Accession # |
NP_003371 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_003380 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
VIM |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100% |
Image 1 | Human brain
| WB Suggested Anti-VIM Antibody Titration: 0.2-1 ug/ml Positive Control: Human brain |
|
Image 2 | Mouse Small Intestine
| Host: Mouse Target Name: VIM Sample Tissue: Mouse Small Intestine Antibody Dilution: 1ug/ml |
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Image 3 | Human Ovary
| Rabbit Anti-VIM Antibody Catalog Number: ARP48225_P050 Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
|
Image 4 | Human COS7
| submitted by:Carl LundinStanford UniversityThe Vimentin antibody ARP48225_P050 works well for visualization of the vimentin cytoskeleton (see attached images). We used PFA-fixed COS-7 cells and an antibody dilution of 1:400. |
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Image 5 | Human brain stem
| Sample Type: Human brain stem cellsPrimary Antibody Dilution: 1:500Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution: 1:1000Color/Signal Descriptions: VIM: Red DAPI:BlueGene Name: VIMSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science |
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