| Product Number |
ARP48176_P050 |
| Product Page |
www.avivasysbio.com/eif3e-antibody-middle-region-arp48176-p050.html |
| Name |
EIF3E Antibody - middle region (ARP48176_P050) |
| Protein Size (# AA) |
445 amino acids |
| Molecular Weight |
52kDa |
| Subunit |
E |
| NCBI Gene Id |
3646 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Eukaryotic translation initiation factor 3, subunit E |
| Alias Symbols |
INT6, EIF3S6, EIF3-P48, eIF3-p46 |
| Peptide Sequence |
Synthetic peptide located within the following region: LNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTK |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Buchsbaum,S., (2007) Oncogene 26 (35), 5132-5144 |
| Description of Target |
EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD); It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. |
| Protein Interactions |
UBC; C4orf19; RUNDC3A; HUWE1; TRIM63; TRIM55; STAU1; SUMO1; NEDD8; GPRASP2; LARP1; GNB2L1; RPS28; RPS25; RPS23; RPS20; RPS19; RPS18; RPS12; RPS10; RPS9; RPS8; RPS3A; RPS3; RPS2; EIF3CL; DYNC1LI1; FBXO6; EIF3D; IGSF8; NPM1; ICAM1; CD81; EIF3B; VCAM1; POLR2 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-EIF3E (ARP48176_P050) antibody |
| Blocking Peptide |
For anti-EIF3E (ARP48176_P050) antibody is Catalog # AAP48176 (Previous Catalog # AAPP28685) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human EIF3E |
| Uniprot ID |
P60228 |
| Protein Name |
Eukaryotic translation initiation factor 3 subunit E |
| Protein Accession # |
NP_001559 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001568 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
EIF3E |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Hela Whole Cell
 | Host: Rabbit
Target Name: EIF3E
Sample Tissue: Human Hela Whole Cell
Antibody Dilution: 1ug/ml |
|
| Image 2 | Human heart
 | Rabbit Anti-EIF3E Antibody
Catalog Number: ARP48176_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic, nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
| Image 3 | Human HepG2
 | WB Suggested Anti-EIF3E Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate |
|
| Image 4 | Human, Mouse
 | Sample Type: 1. B8 mouse fibroblast cells, extraction only2. Human total cell extractPrimary Dilution: 1:1000Secondary Anitbody: HRP conjugated anti-rabbitImage Submitted By: Dr. Elah PickUniversity of Haifa at Oranim |
|
| Image 5 | Human Fetal Lung
 | Host: Rabbit
Target Name: EIF3E
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml |
|
