| Product Number |
ARP48175_P050 |
| Product Page |
www.avivasysbio.com/eif3e-antibody-n-terminal-region-arp48175-p050.html |
| Name |
EIF3E Antibody - N-terminal region (ARP48175_P050) |
| Protein Size (# AA) |
445 amino acids |
| Molecular Weight |
52kDa |
| Subunit |
E |
| NCBI Gene Id |
3646 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Eukaryotic translation initiation factor 3, subunit E |
| Alias Symbols |
INT6, EIF3S6, EIF3-P48, eIF3-p46 |
| Peptide Sequence |
Synthetic peptide located within the following region: ELLQGKLDLLSDTNMVDFAMDVYKNLYSDDIPHALREKRTTVVAQLKQLQ |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Buchsbaum,S., (2007) Oncogene 26 (35), 5132-5144 |
| Description of Target |
EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD); It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. |
| Protein Interactions |
UBC; C4orf19; RUNDC3A; HUWE1; TRIM63; TRIM55; STAU1; SUMO1; NEDD8; GPRASP2; LARP1; GNB2L1; RPS28; RPS25; RPS23; RPS20; RPS19; RPS18; RPS12; RPS10; RPS9; RPS8; RPS3A; RPS3; RPS2; EIF3CL; DYNC1LI1; FBXO6; EIF3D; IGSF8; NPM1; ICAM1; CD81; EIF3B; VCAM1; POLR2 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-EIF3E (ARP48175_P050) antibody |
| Additional Information |
IHC Information: Paraffin embedded kidney tissue, tested with an antibody dilution of 5 ug/ml. |
| Blocking Peptide |
For anti-EIF3E (ARP48175_P050) antibody is Catalog # AAP48175 (Previous Catalog # AAPP28684) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human EIF3E |
| Uniprot ID |
P60228 |
| Protein Name |
Eukaryotic translation initiation factor 3 subunit E |
| Sample Type Confirmation |
EIF3E is supported by BioGPS gene expression data to be expressed in HEK293T |
| Protein Accession # |
NP_001559 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001568 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
EIF3E |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 75% |
| Image 1 | Human 293T
 | WB Suggested Anti-EIF3E Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysateEIF3E is supported by BioGPS gene expression data to be expressed in HEK293T |
|
| Image 2 | Human, Mouse
 | EIF3E antibody - N-terminal region (ARP48175_P050) validated by WB using B8 mouse cells and HEK293 human cells at 1: 1,000. |
|
| Image 3 | Human Kidney
 | Kidney |
|
