P4HB Antibody - C-terminal region (ARP48151_P050)

• Product Number: ARP48151_P050
• Name: P4HB Antibody - C-terminal region (ARP48151_P050)
• Protein Size (# AA): 508 amino acids
• Molecular Weight: 55kDa
• NCBI Gene Id: 5034
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Prolyl 4-hydroxylase, beta polypeptide
• Alias Symbols: DSI, GIT, PDI, PHDB, PDIA1, PO4DB, PO4HB, PROHB, CLCRP1, ERBA2L, P4Hbeta
• Peptide Sequence: Synthetic peptide located within the following region: DRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDD
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)
• Description of Target: P4HB is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: P4HB; UBC; AGO2; MDM2; SUZ12; RNF2; EZH2; BMI1; rev; ACAA2; PLIN3; UBA2; USP5; TPD52L2; PSMD9; PFDN2; CLNS1A; TCEAL4; P3H1; UBQLN2; PABPC1; CALU; PARK2; HNRNPD; EGFR; UBD; BAG3; CXCR4; env; UBL4A; CSNK2A2; ATF2; GRDX; TMED9; GANAB; CCT4; CCT7; PDIA6; PDIA
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-P4HB (ARP48151_P050) antibody is Catalog # AAP48151 (Previous Catalog # AAPP28662)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human P4HB
• Uniprot ID: P07237
• Protein Name: Protein disulfide-isomerase
• Sample Type Confirmation:

  P4HB is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: NP_000909
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000918
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: P4HB
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Mouse Small Intestine
  
  Host: Mouse
  Target Name: P4HB
  Sample Tissue: Mouse Small Intestine
  Antibody Dilution: 1ug/ml
• Image 2: Human Lung Tissue
  
  P4HB antibody - C-terminal region (ARP48151_P050)
  Catalog Number: ARP48151_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
  Observed Staining: Cytoplasm of pneumocytes
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 3: Human Liver Tissue
  
  P4HB antibody - C-terminal region (ARP48151_P050)
  Catalog Number: ARP48151_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
  Observed Staining: Cytoplasm in hepatocytes
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 4: Human HepG2
  
  WB Suggested Anti-P4HB Antibody Titration: 0.2-1 ug/ml
  Positive Control: HepG2 cell lysateP4HB is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells