Product Number |
ARP48133_P050 |
Product Page |
www.avivasysbio.com/hadhb-antibody-c-terminal-region-arp48133-p050.html |
Name |
HADHB Antibody - C-terminal region (ARP48133_P050) |
Protein Size (# AA) |
474 amino acids |
Molecular Weight |
47kDa |
Subunit |
beta, mitochondrial |
NCBI Gene Id |
3032 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit |
Description |
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Alias Symbols |
ECHB, MTPB, MSTP029, TP-BETA |
Peptide Sequence |
Synthetic peptide located within the following region: LLLGPTYATPKVLEKAGLTMNDIDAFEFHEAFSGQILANFKAMDSDWFAE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Wang,R., (2006) Zhonghua Fu Chan Ke Za Zhi 41 (10), 672-675 |
Description of Target |
HADHB is the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. Mutations in HADHB gene result in trifunctional protein deficiency. The protein can also bind RNA and decreases the stability of some mRNAs.This gene encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. Mutations in this gene result in trifunctional protein deficiency. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Alternatively spliced transcript variants have been found; however, their full-length nature is not known. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Protein Interactions |
HUWE1; TP53; TUBG1; SUMO2; SUMO3; UBC; PARK2; PPP6R1; ADRB2; CSNK2A2; PAN2; ATF2; vpu; TIMM8B; COX17; IQCB1; SUOX; CHCHD4; TUBB1; RPS8; OPA1; HSP90AB1; HADHA; DES; GRK5; FBXO6; CDK2; LEO1; RTF1; TK1; SMN1; PSMA3; GRB7; RCC1; CDKN1A; ANXA7; Edc4; MYC; SQST |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
Relative Expression (Western Blot) |
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Datasheets/Manuals |
Printable datasheet for anti-HADHB (ARP48133_P050) antibody |
Additional Information |
IHC Information: Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE) IHC Information: Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) |
Blocking Peptide |
For anti-HADHB (ARP48133_P050) antibody is Catalog # AAP48133 (Previous Catalog # AAPP28649) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human HADHB |
Uniprot ID |
P55084 |
Protein Name |
Trifunctional enzyme subunit beta, mitochondrial |
Publications |
Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy. J Mol Cell Cardiol. 138, 304-317 (2020). 31836543
Gerin, I. et al. Expression of miR-33 from an SREBP2 intron inhibits cholesterol export and fatty acid oxidation. J. Biol. Chem. 285, 33652-61 (2010). 20732877
The nuclear receptor FXR uncouples the actions of miR-33 from SREBP-2. Arterioscler Thromb Vasc Biol. 35, 787-95 (2015). 25593129
TXNIP regulates myocardial fatty acid oxidation via miR-33a signaling. Am. J. Physiol. Heart Circ. Physiol. 311, H64-75 (2016). 27199118 |
Sample Type Confirmation |
HADHB is supported by BioGPS gene expression data to be expressed in HepG2 |
Protein Accession # |
NP_000174 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_000183 |
Tested Species Reactivity |
Human |
Gene Symbol |
HADHB |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Yeast, Zebrafish |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%; Yeast: 83%; Zebrafish: 86% |
Image 1 |
| 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
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