MAPT Antibody - middle region (ARP48103_P050)

Data Sheet
 
Product Number ARP48103_P050
Product Page www.avivasysbio.com/mapt-antibody-middle-region-arp48103-p050.html
Name MAPT Antibody - middle region (ARP48103_P050)
Protein Size (# AA) 758 amino acids
Molecular Weight 79 kDa
NCBI Gene Id 4137
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Microtubule-associated protein tau
Alias Symbols TAU, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, tau-40, FTDP-17, PPP1R103
Peptide Sequence Synthetic peptide located within the following region: RGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPG
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Reynolds,M.R., (2006) Biochemistry 45 (13), 4314-4326
Description of Target MAPT is differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. The mutations in the gene have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Protein Interactions Ywhaz; Prkaca; STUB1; PHGK; SRC; PRKAR1A; GSK3B; FYN; DCTN1; CSNK1A1; CDK2; AKT1; ABL1; YWHAQ; SGK1; MARK2; MARCH7; TUBB3; TUBA1B; UBC; TUBA4A; MARK4; PEG10; MARK3; MARK1; MAPK13; MAPK11; MAPK8; mapk14; Mapk12; LIMS1; HSPA1A; PPP5C; PPP2R4; CAPN2; PKN1; H
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-MAPT (ARP48103_P050) antibody
Blocking Peptide For anti-MAPT (ARP48103_P050) antibody is Catalog # AAP48103 (Previous Catalog # AAPS21802)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human MAPT
Uniprot ID P10636-8
Protein Name Microtubule-associated protein tau
Protein Accession # NP_005901
Purification Affinity Purified
Nucleotide Accession # NM_005910
Tested Species Reactivity Human, Mouse
Gene Symbol MAPT
Predicted Species Reactivity Human, Mouse, Rat, Pig
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Human: 100%; Mouse: 86%; Pig: 79%; Rat: 86%
Image 1
E.Coli: expressed human Tau-C, E.Coli: expressed human Tau-E, E.Coli: expressed human Tau-F, E.Coli: expressed human Tau-B, E.Coli: expressed human Fetal-Tau, E.Coli: expressed human Tau-D
Researcher: Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig
Application: Western blotting
Species + Tissue/Cell type:
Lane 1: 10ug E.Coli: expressed human Tau-C
Lane 2: 10ug E.Coli: expressed human Tau-E
Lane 3: 10ug E.Coli: expressed human Tau-F
Lane 4: 10ug E.Coli: expressed human Tau-B
Lane 5: 10ug E.Coli: expressed human Fetal-Tau
Lane 6: 10ug E.Coli: expressed human Tau-D
Primary antibody dilution: 1:1000
Secondary antibody: Donkey anti-rabbit-HRP
Secondary antibody dilution: 1:10,000
Image 2
mouse cortex
Sample Type:
A: Transgenic hTau P301L mouse cortex B: Tau-Ko mouse cortex C: WT mouse cortex
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-biotin / Avidin-HRP
Secondary Antibody Dilution:
1:1000 /1:2000
Color/Signal Descriptions:
Dark grey: MAPT
Gene Name:
MAPT
Submitted by:
Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig
Image 3

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The peptide sequence is present in a 45 kDa isoform. The protein may be modified by acetylation, glycosylation, and phosphorylation.
 

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