| Product Number |
ARP48061_P050 |
| Product Page |
https://www.avivasysbio.com/akt1s1-antibody-c-terminal-region-arp48061-p050.html |
| Name |
AKT1S1 Antibody - C-terminal region (ARP48061_P050) |
| Protein Size (# AA) |
256 amino acids |
| Molecular Weight |
27kDa |
| NCBI Gene Id |
84335 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
AKT1 substrate 1 (proline-rich) |
| Alias Symbols |
Lobe, PRAS40 |
| Peptide Sequence |
Synthetic peptide located within the following region: KSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAE |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Fonseca,B.D., (2008) Biochem. J. 411 (1), 141-149 |
| Description of Target |
AKT1S1 may play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. It is the substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. AKT1S1 may also play a role in nerve growth factor-mediated neuroprotection.AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]. |
| Protein Interactions |
MTOR; RPTOR; SGK1; RICTOR; MAPKAP1; MLST8; FAM114A1; YWHAQ; YWHAB; SQSTM1; RPS6KA5; TCEA2; YWHAG; EIF6; AKT2; AKT1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-AKT1S1 (ARP48061_P050) antibody |
| Blocking Peptide |
For anti-AKT1S1 (ARP48061_P050) antibody is Catalog # AAP48061 (Previous Catalog # AAPY02091) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human AKT1S1 |
| Uniprot ID |
Q96B36 |
| Protein Name |
Proline-rich AKT1 substrate 1 |
| Protein Accession # |
NP_115751 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_032375 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
AKT1S1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100% |
| Image 1 | Human heart
 | Rabbit Anti-AKT1S1 Antibody
Catalog Number: ARP48061_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic in endothelial cells in blood vessels
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
| Image 2 | Human 293T
 | WB Suggested Anti-AKT1S1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 293T cell lysate |
|
