CKAP4 Antibody - middle region (ARP46790_P050)

• Product Number: ARP46790_P050
• Name: CKAP4 Antibody - middle region (ARP46790_P050)
• Protein Size (# AA): 602 amino acids
• Molecular Weight: 66kDa
• NCBI Gene Id: 10970
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Cytoskeleton-associated protein 4
• Alias Symbols: p63, CLIMP63, CLIMP-63, ERGIC-63
• Peptide Sequence: Synthetic peptide located within the following region: SDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ko,C.J., (2008) J. Cutan. Pathol. 35 (4), 353-357
• Description of Target: CKAP4/p63 is a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and p63 were useful in identifying squamous cell carcinomas with single cell infiltration. Those results suggested a role of CKAP4/p63 in tumor.
• Protein Interactions: FUS; UBC; TP53; SPRTN; SUMO1; NEDD8; MDM2; PARK2; ADRA1D; THOC7; HAMP; env; CCDC53; CKAP4; MVP; TSNAX; NKX3-1; NDUFB7; MYC; MLH1; ATF4; ECT2; COPS5; CUL3; SH3KBP1; ARRB1; ELAVL1; HDGF; CLN3; USP50; ASCC2; PLAT;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Additional Information: IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml.
• Blocking Peptide: For anti-CKAP4 (ARP46790_P050) antibody is Catalog # AAP46790 (Previous Catalog # AAPP27588)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CKAP4
• Uniprot ID: Q07065
• Protein Name: Cytoskeleton-associated protein 4
• Publications:

  Clark, R. et al. Comparative genomic and proteomic analysis of cytoskeletal changes in dexamethasone-treated trabecular meshwork cells. Mol. Cell. Proteomics 12, 194-206 (2013). 23105009

• Sample Type Confirmation:

  CKAP4 is supported by BioGPS gene expression data to be expressed in HeLa

• Protein Accession #: NP_006816
• Purification: Affinity Purified
• Nucleotide Accession #: NM_006825
• Tested Species Reactivity: Human
• Gene Symbol: CKAP4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100%
• Image 1: Hela, MCF7
  
  Host: Rabbit
  Target: CKAP4
  Positive control (+): Hela (HL)
  Negative control (-): MCF7 (N10)
  Antibody concentration: 1ug/ml
• Image 2: Human Hela Whole Cell
  
  Host: Rabbit
  Target Name: CKAP4
  Sample Tissue: Human Hela Whole Cell
  Antibody Dilution: 1ug/ml
• Image 3: Human Breast
  
  Anti-CKAP4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
• Image 4: Human Hela Whole Cell
  
  Host: Rabbit
  Target Name: CKAP4
  Sample Tissue: Human Hela Whole Cell
  Antibody Dilution: 1ug/ml
• Image 5: Human Bronchial Epithelial Tissue
  
  Rabbit Anti-CKAP4 Antibody
  Catalog Number: ARP46790_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
  Observed Staining: Membrane and cytoplasmic in excellent staining !
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1/600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 6: Human Hela
  
  Host: Rabbit
  Target Name: CKAP4
  Sample Tissue: Human Hela
  Antibody Dilution: 1.0ug/ml