VARS Antibody - middle region (ARP46151_P050)

• Product Number: ARP46151_P050
• Name: VARS Antibody - middle region (ARP46151_P050)
• Protein Size (# AA): 1264 amino acids
• Molecular Weight: 140kDa
• NCBI Gene Id: 7407
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Valyl-tRNA synthetase
• Alias Symbols: G7A, VARS, VARS2, NDMSCA
• Peptide Sequence: Synthetic peptide located within the following region: VFDEFVDMDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)
• Description of Target: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. VARS belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: CALCOCO2; SUMO2; SART3; STAU1; UBC; EED; CCT4; KNTC1; AIMP1; CCT3; QARS; EEF1G; EEF1D; EEF1B2; FBXO6; YWHAQ; MAPK11; VCAM1; ITGA4; FN1; LARS; CAD; UBD; SHC1; CUL3; CDK2; CHD7; CACNA1A; ATG101; OTUD5; DMWD; EEF1A1; EEF1A2; TRAF6; TFE3; HLA-B; EPB41;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-VARS (ARP46151_P050) antibody is Catalog # AAP46151 (Previous Catalog # AAPS17009)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human VARS
• Uniprot ID: P26640
• Protein Name: Valine--tRNA ligase
• Publications:

  Duarte, M. & Videira, A. Defective valyl-tRNA synthetase hampers the mitochondrial respiratory chain in Neurospora crassa. Biochem. J. 448, 297-306 (2012). 22957697

• Sample Type Confirmation:

  VARS is strongly supported by BioGPS gene expression data to be expressed in 721_B

• Protein Accession #: NP_006286
• Purification: Affinity Purified
• Nucleotide Accession #: NM_006295
• Tested Species Reactivity: Human
• Gene Symbol: VARS
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 86%; Zebrafish: 86%
• Image 1: Human 721_B
  
  WB Suggested Anti-VARS Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: 721_B cell lysateVARS is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
• Image 2: Human Lung Tissue
  
  VARS antibody - middle region (ARP46151_P050)
  Catalog Number: ARP46151_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
  Observed Staining: Cytoplasm of pneumocytes
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1/600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec