Product Number |
ARP46096_P050 |
Product Page |
www.avivasysbio.com/fen1-antibody-n-terminal-region-arp46096-p050.html |
Name |
FEN1 Antibody - N-terminal region (ARP46096_P050) |
Protein Size (# AA) |
380 amino acids |
Molecular Weight |
42kDa |
NCBI Gene Id |
2237 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Flap structure-specific endonuclease 1 |
Alias Symbols |
MF1, RAD2, FEN-1 |
Peptide Sequence |
Synthetic peptide located within the following region: APSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSH |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. |
Protein Interactions |
SUMO2; SUMO3; PCNA; UBC; SHFM1; ELAC2; TNPO3; PPP2R5D; HARS; LPP; PFDN1; WRN; AICDA; RAD1; FN1; PAXIP1; APP; VRK1; RAP1A; cdt1; TERF1; RAD21; GSK3B; ENDOG; DDX11; NCAPG; EP300; BLM; EEF1G; ARHGDIA; HNRNPA1; CDK2; CDK1; APEX1; RAD9A; HUS1; CCNA2; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-FEN1 (ARP46096_P050) antibody |
Blocking Peptide |
For anti-FEN1 (ARP46096_P050) antibody is Catalog # AAP46096 |
Uniprot ID |
P39748 |
Protein Name |
Flap endonuclease 1 |
Sample Type Confirmation |
FEN1 is supported by BioGPS gene expression data to be expressed in HEK293T |
Protein Accession # |
NP_004102 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_004111 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
FEN1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Sheep, Zebrafish |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%; Sheep: 100%; Zebrafish: 79% |
Image 1 | Human 293T
| WB Suggested Anti-FEN1 Antibody Titration: 1.0 ug/ml Positive Control: 293T Whole CellFEN1 is supported by BioGPS gene expression data to be expressed in HEK293T |
|
Image 2 | BL21
| WB Suggested Anti-FEN1 Antibody Positive Control: Lane1: hFEN1 (1-336), Lane2: uninduced BL21, Lane3: 2h induced BL21, Lane4: overnight induced BL21 Primary Antibody Dilution : 1:2000 Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:10,000 Submitted by: Prof. Jon R Sayers, University of Sheffield Medical School |
|
Image 3 | Mouse Testis
| Host: Mouse Target Name: FEN1 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml |
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