FEN1 Antibody - N-terminal region (ARP46096_P050)

Data Sheet
 
Product Number ARP46096_P050
Product Page www.avivasysbio.com/fen1-antibody-n-terminal-region-arp46096-p050.html
Name FEN1 Antibody - N-terminal region (ARP46096_P050)
Protein Size (# AA) 380 amino acids
Molecular Weight 42kDa
NCBI Gene Id 2237
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Flap structure-specific endonuclease 1
Alias Symbols MF1, RAD2, FEN-1
Peptide Sequence Synthetic peptide located within the following region: APSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSH
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Protein Interactions SUMO2; SUMO3; PCNA; UBC; SHFM1; ELAC2; TNPO3; PPP2R5D; HARS; LPP; PFDN1; WRN; AICDA; RAD1; FN1; PAXIP1; APP; VRK1; RAP1A; cdt1; TERF1; RAD21; GSK3B; ENDOG; DDX11; NCAPG; EP300; BLM; EEF1G; ARHGDIA; HNRNPA1; CDK2; CDK1; APEX1; RAD9A; HUS1; CCNA2;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-FEN1 (ARP46096_P050) antibody
Blocking Peptide For anti-FEN1 (ARP46096_P050) antibody is Catalog # AAP46096
Uniprot ID P39748
Protein Name Flap endonuclease 1
Sample Type Confirmation

FEN1 is supported by BioGPS gene expression data to be expressed in HEK293T

Protein Accession # NP_004102
Purification Affinity Purified
Nucleotide Accession # NM_004111
Tested Species Reactivity Human, Mouse
Gene Symbol FEN1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Sheep, Zebrafish
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%; Sheep: 100%; Zebrafish: 79%
Image 1
Human 293T
WB Suggested Anti-FEN1 Antibody
Titration: 1.0 ug/ml
Positive Control: 293T Whole CellFEN1 is supported by BioGPS gene expression data to be expressed in HEK293T
Image 2
BL21
WB Suggested Anti-FEN1 Antibody
Positive Control: Lane1: hFEN1 (1-336), Lane2: uninduced BL21, Lane3: 2h induced BL21, Lane4: overnight induced BL21
Primary Antibody Dilution : 1:2000
Secondary Antibody : Goat anti-rabbit-HRP
Secondry Antibody Dilution : 1:10,000
Submitted by: Prof. Jon R Sayers, University of Sheffield Medical School
Image 3
Mouse Testis
Host: Mouse
Target Name: FEN1
Sample Tissue: Mouse Testis
Antibody Dilution: 1ug/ml
 

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