PSMB9 Antibody - C-terminal region (ARP46081_P050)

• Product Number: ARP46081_P050
• Name: PSMB9 Antibody - C-terminal region (ARP46081_P050)
• Protein Size (# AA): 219 amino acids
• Molecular Weight: 23 kDa
• Subunit: beta type-9
• NCBI Gene Id: 5698
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
• Alias Symbols: LMP2, PRAAS3, PSMB6i, RING12, beta1i
• Peptide Sequence: Synthetic peptide located within the following region: RRFTTDAIALAMSRDGSSGGVIYLVTITAAGVDHRVILGNELPKFYDE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Deshpande,A., (2008) J. Infect. Dis. 197 (3), 371-381
• Description of Target: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMB9 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This subunit is involved in antigen processing to generate class I binding peptides. Expression of PSMB9 is induced by gamma interferon and it replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding different isoforms have been identified; both isoforms are processed to yield the same mature subunit.
• Protein Interactions: NCOA3; TCEB3; SRC; POLR2L; POLR2K; POLR2J; POLR2I; POLR2H; POLR2G; POLR2F; POLR2E; POLR2D; POLR2C; POLR2B; ESR1; NCOA2; UBC; HCVgp1; PSMA2; PSMB5; PSME2; PSME1; PSMB6; PSMA3; POMP; PSMB8; PSMB7; PSMA7; PSMA1; PSMB9; PSMC5; PSMA4; POLR2A; NCOA1; PSMB10;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-PSMB9 (ARP46081_P050) antibody is Catalog # AAP46081 (Previous Catalog # AAPP26943)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PSMB9
• Uniprot ID: P28065
• Protein Name: Proteasome subunit beta type-9
• Sample Type Confirmation:

  PSMB9 is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_002791
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002800
• Tested Species Reactivity: Human
• Gene Symbol: PSMB9
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Image 1:
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.