| Product Number |
ARP46079_P050 |
| Product Page |
www.avivasysbio.com/ppat-antibody-n-terminal-region-arp46079-p050.html |
| Name |
PPAT Antibody - N-terminal region (ARP46079_P050) |
| Protein Size (# AA) |
517 amino acids |
| Molecular Weight |
57 kDa |
| NCBI Gene Id |
5471 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Phosphoribosyl pyrophosphate amidotransferase |
| Alias Symbols |
GPAT, PRAT, ATASE |
| Peptide Sequence |
Synthetic peptide located within the following region: VTSDGSSVPTFKSHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKC |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Bera,A.K., (1999) J. Biol. Chem. 274 (51), 36498-36504 |
| Description of Target |
PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. Its gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4. |
| Protein Interactions |
FAM96B; CIAO1; FAM114A1; PARVA; CARM1; RANBP3; TROVE2; RSU1; MEA1; ILK; HSPA4; GART; CARS; MMS19; EPT1; ATXN1; SMURF1; MCM3; UBC; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Enhanced Validation |
| Relative Expression (Western Blot) |
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| Datasheets/Manuals |
Printable datasheet for anti-PPAT (ARP46079_P050) antibody |
| Additional Information |
IHC Information: Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. |
| Blocking Peptide |
For anti-PPAT (ARP46079_P050) antibody is Catalog # AAP46079 (Previous Catalog # AAPS17401) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human PPAT |
| Uniprot ID |
Q06203 |
| Protein Name |
Amidophosphoribosyltransferase |
| Sample Type Confirmation |
PPAT is supported by BioGPS gene expression data to be expressed in HepG2 |
| Protein Accession # |
NP_002694 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_002703 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
PPAT |
| Predicted Species Reactivity |
Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 86%; Dog: 86%; Guinea Pig: 86%; Horse: 86%; Human: 100%; Rabbit: 93%; Rat: 79% |
| Image 1 | Human kidney
 | Human kidney |
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| Image 2 | Human, Hamster
 | Sample Type: 1. Human Skin Fibroblasts (100ug)
2. HepG2 cells (20ug)
3. HEK273 cells (20ug)
4. HeLa skin cells(20ug)
5. Hamster CHO K1 cells (20ug)
Primary Dilution: 1:5000
Secondary Antibody: Clean-Blot IP detection Reagent and Kit
Secondary Dilution: 1:2000
Image Submitted By: V. Skopova, M. Zikanova
Institute of Inherited Metabolic Disorders, Charles University in Prague and General Hopspital in Prague
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| Image 3 |
 | 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.
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