TDO2 Antibody - N-terminal region (ARP45716_P050)

Data Sheet
 
Product Number ARP45716_P050
Product Page www.avivasysbio.com/tdo2-antibody-n-terminal-region-arp45716-p050.html
Name TDO2 Antibody - N-terminal region (ARP45716_P050)
Protein Size (# AA) 799 amino acids
Molecular Weight 48kDa
NCBI Gene Id 6999
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Tryptophan 2,3-dioxygenase
Alias Symbols TO, TDO, TPH2, TRPO, HYPTRP
Peptide Sequence Synthetic peptide located within the following region: MSGCPFLGNNFGYTFKKLPVEGSEEDKSQTGVNRASKGGLIYGNYLHLEK
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Blaschke,S., (2002) Int. J. Cancer 101 (4), 327-334
Description of Target Tryptophan 2,3-dioxygenase (EC 1.13.11.11) plays a role in catalyzing the first and rat-limiting step in the kynurenine pathway, the major pathway of tryptophan metabolism.
Protein Interactions MOB3C; NGB; MOB1A; SDCBP2; ZFYVE26; DPM1; ASMTL; TDO2; SDCBP; PSMA1; EIF4E; NMNAT1; REL; MEOX2; PRMT6; KDM1A;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-TDO2 (ARP45716_P050) antibody
Blocking Peptide For anti-TDO2 (ARP45716_P050) antibody is Catalog # AAP45716 (Previous Catalog # AAPP11849)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TDO2
Uniprot ID P48775
Protein Name Tryptophan 2,3-dioxygenase
Protein Accession # NP_005642
Purification Affinity Purified
Nucleotide Accession # NM_005651
Tested Species Reactivity Human
Gene Symbol TDO2
Predicted Species Reactivity Human, Dog, Pig
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Dog: 79%; Human: 100%; Pig: 92%
Image 1
Human Jurkat Whole Cell
Host: Rabbit
Target Name: TDO2
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 5ug/ml
Image 2
Human 786-0 Whole Cell
Host: Rabbit
Target Name: TDO2
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 1ug/ml
Image 3
Human 293T Whole Cell
Host: Rabbit
Target Name: TDO2
Sample Tissue: Human 293T Whole Cell
Antibody Dilution: 1ug/ml
Image 4
Human Fetal Heart
WB Suggested Anti-TDO2 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Human Fetal Heart
Image 5
Human Kidney
Anti-TDO2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody ARP45716_P050 concentration 5 ug/ml.
Image 6

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
 

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