EPOR Antibody - N-terminal region (ARP44216_P050)

• Product Number: ARP44216_P050
• Name: EPOR Antibody - N-terminal region (ARP44216_P050)
• Protein Size (# AA): 508 amino acids
• Molecular Weight: 53kDa
• NCBI Gene Id: 2057
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Erythropoietin receptor
• Alias Symbols: EPO-R
• Peptide Sequence: Synthetic peptide located within the following region: DHLGASLWPQVGSLCLLLAGAAWAPPPNLPDPKFESKAALLAARGPEELL
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Sinclair,A.M., (2008) Br. J. Cancer 98 (6), 1059-1067
• Description of Target: The erythropoietin receptor is a member of the cytokine receptor family. Upon erythropoietin binding, the erythropoietin receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis.The erythropoietin receptor is a member of the cytokine receptor family. Upon erythropoietin binding, the erythropoietin receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: PIK3R1; SRC; MST1R; JAK2; SH2B2; STAT5A; CBL; CRKL; GRB2; UBC; SOCS2; PLCG1; INPP5D; CISH; NOSIP; ATXN2L; GAB2; GAB1; GRAP; STAT5B; SOCS3; MAPK3; IRS2; SHC1; PLCG2; SYK; KITLG; GNB2L1; MAPK1; PTPN6; PTPN11; KLK3; LYN; KIT; VAV1; GNAI1; CSF2RB; EPOR; EPO;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-EPOR (ARP44216_P050) antibody is Catalog # AAP44216 (Previous Catalog # AAPP11992)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EPOR
• Uniprot ID: P19235
• Protein Name: Erythropoietin receptor
• Protein Accession #: NP_000112
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000121
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: EPOR
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 85%; Dog: 100%; Goat: 91%; Guinea Pig: 93%; Horse: 92%; Human: 100%; Mouse: 100%; Rat: 100%
• Image 1: Human Brain
  
  WB Suggested Anti-EPOR Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Human brain
• Image 2: Human Bone Marrow
  
  Rabbit Anti-EPOR Antibody
  Catalog Number: ARP44216_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue
  Observed Staining: Cytoplasm, Plasma membrane
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 3: Mouse Small Intestine
  
  Host: Mouse
  Target Name: EPOR
  Sample Tissue: Mouse Small Intestine
  Antibody Dilution: 1ug/ml
• Image 4: Mouse Small Intestine
  
  Host: Rabbit
  Target Name: EPOR
  Sample Tissue: Mouse Small Intestine
  Antibody Dilution: 1ug/ml
• Image 5:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.