TAP1 Antibody - middle region (ARP43628_P050)

• Product Number: ARP43628_P050
• Name: TAP1 Antibody - middle region (ARP43628_P050)
• Protein Size (# AA): 808 amino acids
• Molecular Weight: 87 kDa
• NCBI Gene Id: 6890
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• Alias Symbols: APT1, PSF1, ABC17, ABCB2, PSF-1, RING4, TAP1N, D6S114E, TAP1*0102N
• Peptide Sequence: Synthetic peptide located within the following region: LVTFVLYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFEYLDRTPRCPPSGL
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Soundravally,R. (2008) Scand. J. Immunol. 67 (6), 618-625
• Description of Target: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). TAP1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. TAP1 is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble.The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: UBC; MUL1; TDP2; SFXN1; GADD45GIP1; MRPL40; MCAT; SRSF3; SDHA; ABCD3; CYC1; PSMB8; TAPBP; TAP2; HLA-A; PDIA3; CALR; B2M; PDIA2; KRTAP4-12; MDFI; PSMB5; HLA-F; HLA-G; ESR1; COPG1; COPB1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-TAP1 (ARP43628_P050) antibody is Catalog # AAP43628 (Previous Catalog # AAPP11617)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TAP1
• Uniprot ID: Q03518
• Protein Name: Antigen peptide transporter 1
• Sample Type Confirmation:

  TAP1 is supported by BioGPS gene expression data to be expressed in HeLa

• Protein Accession #: NP_000584
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000593
• Tested Species Reactivity: Human
• Gene Symbol: TAP1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100%; Zebrafish: 83%
• Image 1: Human Ovary, HepG2
  
  Host: Rabbit
  Target: TAP1
  Positive control (+): Human Ovary (OV)
  Negative control (-): HepG2 (HG)
  Antibody concentration: 4ug/ml
• Image 2: Human Lung Tissue
  
  Rabbit Anti-TAP1 Antibody
  Catalog Number: ARP43628_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
  Observed Staining: Cytoplasmic in alveolar type I & II cells
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 3: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 4 ug/mL of the antibody was used in this experiment. Recommended dilution for this antibody is 1-3 ug/mL.