ECH1 Antibody - N-terminal region (ARP43560_T100)

• Product Number: ARP43560_T100
• Name: ECH1 Antibody - N-terminal region (ARP43560_T100)
• Protein Size (# AA): 328 amino acids
• Molecular Weight: 36kDa
• NCBI Gene Id: 1891
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Enoyl CoA hydratase 1, peroxisomal
• Alias Symbols: HPXEL
• Peptide Sequence: Synthetic peptide located within the following region: PDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Goehler,H., (2004) Mol. Cell 15 (6), 853-865
• Description of Target: ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The protein contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway.This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
• Protein Interactions: FAM9B; HUWE1; TRAF1; UBC; MDM2; PPP6R3; IQCB1; STAT3; PEX5; EEF1D; DNAJB11; HNRNPUL1; OPA1; APP; HTT; EEF1G; HDAC5; ICT1; TLR10; PAFAH1B3; SERPINB9; CSTF2; TIMP1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-ECH1 (ARP43560_T100) antibody is Catalog # AAP43560 (Previous Catalog # AAPP25005)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ECH1
• Uniprot ID: Q13011
• Protein Name: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• Publications:

  Xie, W., Zhang, S., Lei, F., Ouyang, X. & Du, L. Ananas comosus L. Leaf Phenols and p-Coumaric Acid Regulate Liver Fat Metabolism by Upregulating CPT-1 Expression. Evid. Based. Complement. Alternat. Med. 2014, 903258 (2014). 25197313

  Xie, W.-D. et al. Enhanced peroxisomal beta-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice. Exp. Ther. Med. 2, 309-315 (2011). 22977503

• Sample Type Confirmation:

  ECH1 is supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: NP_001389
• Purification: Protein A purified
• Nucleotide Accession #: NM_001398
• Tested Species Reactivity: Human
• Gene Symbol: ECH1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 92%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 86%; Rat: 100%
• Image 1: Human HepG2
  
  WB Suggested Anti-ECH1 Antibody Titration: 2.5ug/ml
  Positive Control: HepG2 cell lysateECH1 is supported by BioGPS gene expression data to be expressed in HepG2