RNF31 Antibody - middle region (ARP43241_P050)

Data Sheet
 
Product Number ARP43241_P050
Product Page www.avivasysbio.com/rnf31-antibody-middle-region-arp43241-p050.html
Name RNF31 Antibody - middle region (ARP43241_P050)
Gene Symbol RNF31
Alias Symbols FLJ10111, FLJ23501, MGC19975, ZIBRA, HOIP
Protein Size (# AA) 1072 amino acids
Molecular Weight 120kDa
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
NCBI Gene Id 55072
Host Rabbit
Clonality Polyclonal
Concentration Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Official Gene Full Name Ring finger protein 31
Peptide Sequence Synthetic peptide located within the following region: SLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKL
Target Reference Kirisako,T., (2006) EMBO J. 25 (20), 4877-4887
Description of Target PWP2 contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.
Protein Interactions CCDC24; VASN; KRTAP9-2; TSSC4; UBC; SNRPC; PRKCA; AES; PITX1; KIF1A; SHARPIN; RBCK1; RNF31; MALT1; VCP; OTULIN; IRAK1; ESR1; Ikbkg; CCDC14; WRNIP1; AKAP8L; TRAF2; TNF; NDUFA8; BIRC2; NOD2; BIRC3; HCVgp1; SOCS3; SMAD3; NOS3; UBE2D3; UBE2D1; TNFAIP3; GFI1B;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-RNF31 (ARP43241_P050) antibody
Blocking Peptide For anti-RNF31 (ARP43241_P050) antibody is Catalog # AAP43241 (Previous Catalog # AAPP26473)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human RNF31
Complete computational species homology data Anti-RNF31 (ARP43241_P050)
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express RNF31.
Swissprot Id Q96EP0
Protein Name E3 ubiquitin-protein ligase RNF31
Publications

Blackwell, K. et al. Two coordinated mechanisms underlie tumor necrosis factor alpha-induced immediate and delayed IκB kinase activation. Mol. Cell. Biol. 33, 1901-15 (2013). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 23459942

Kupka, S; De Miguel, D; Draber, P; Martino, L; Surinova, S; Rittinger, K; Walczak, H; SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes. 16, 2271-80 (2016). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 27545878

Lafont, E; Kantari-Mimoun, C; Draber, P; De Miguel, D; Hartwig, T; Reichert, M; Kupka, S; Shimizu, Y; Taraborrelli, L; Spit, M; Sprick, MR; Walczak, H; The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. , (2017). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 28258062

Liu, S. et al. MAVS recruits multiple ubiquitin E3 ligases to activate antiviral signaling cascades. Elife 2, e00785 (2013). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 23951545

Queisser, M. A. et al. HOIL-1L Functions as the PKCz Ubiquitin Ligase to Promote Lung Tumor Growth. (2014). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 25118570

Shibata, Y; Tokunaga, F; Goto, E; Komatsu, G; Gohda, J; Saeki, Y; Tanaka, K; Takahashi, H; Sawasaki, T; Inoue, S; Oshiumi, H; Seya, T; Nakano, H; Tanaka, Y; Iwai, K; Inoue, JI; HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains. 13, e1006162 (2017). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 28103322

Sieber, S. et al. Sharpin contributes to TNF-alpha dependent NFκB activation and anti-apoptotic signalling in hepatocytes. PLoS One 7, e29993 (2012). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 22253853

Smit, J. J. et al. The E3 ligase HOIP specifies linear ubiquitin chain assembly through its RING-IBR-RING domain and the unique LDD extension. EMBO J. 31, 3833-44 (2012). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 22863777

Protein Accession # NP_060469
Purification Affinity Purified
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express RNF31.
Nucleotide Accession # NM_017999
Replacement Item This antibody may replace item sc-153046 from Santa Cruz Biotechnology.
Tested Species Reactivity Human
Predicted Species Reactivity Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100%
Image 1
Human A549
Lanes:
Lane1: 50 ug hormoxia A549 lysate
Lane2: 50 ug hypoxia A549 lysate
Lane3: 50 ug hormoxia A549 lysate (+scrambled siRNA)
Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA)
Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA)
Lane6: 50 ug hypoxia A549 lysate (RNF31 siRNA)
Primary Antibody Dilution:
1:800
Secondary Antibody:
Goat anti rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
RNF31
Submitted by:
Markus Queisser, Northwestern University
Image 2
Human MCF7
Host: Rabbit
Target Name: RNF31
Sample Tissue: Human MCF7
Antibody Dilution: 1.0ug/ml
 

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