FBXO7 Antibody - middle region (ARP43128_P050)

• Product Number: ARP43128_P050
• Name: FBXO7 Antibody - middle region (ARP43128_P050)
• Protein Size (# AA): 522 amino acids
• Molecular Weight: 59 kDa
• NCBI Gene Id: 25793
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: F-box protein 7
• Description:
  View product citations for FBXO7 Antibody - middle region (ARP43128_P050) on CiteAb
• Alias Symbols: FBX, FBX7, PKPS, FBX07, PARK15
• Peptide Sequence: Synthetic peptide located within the following region: PGETPSQFPPLRPRFDPVGPLPGPNPILPGRGGPNDRFPFRPSRGRPTDG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Chang,Y.F., (2006) Biochem. Biophys. Res. Commun. 342 (4), 1022-1026
• Description of Target: FBXO7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO7 belongs to the Fbxs class and it may play a role in regulation of hematopoiesis.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it may play a role in regulation of hematopoiesis. Alternatively spliced transcript variants of this gene have been identified with the full-length natures of only some variants being determined.
• Protein Interactions: PSME3; PSMF1; PSMA3; SKP1; TRAF2; MAGED1; SHFM1; PINK1; PARK2; SEC24C; NEDD8; UBC; COPS5; COPS6; CUL1; BIRC2; RBBP6; XPO1; DLGAP5; ELF4; BIRC3; CDK6; UBE2D2; CDC34;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Other Applications Image 1 Data: Immunoprecipitation -- 5ug antibody
• Blocking Peptide: For anti-FBXO7 (ARP43128_P050) antibody is Catalog # AAP43128 (Previous Catalog # AAPP25101)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXO7
• Uniprot ID: Q9Y3I1
• Protein Name: F-box only protein 7
• Publications:

  Chemical inhibition of FBXO7 reduces inflammation and confers neuroprotection by stabilizing the mitochondrial kinase PINK1. JCI Insight. 5, (2020). 32493843

  The Parkinson's disease-linked proteins Fbxo7 and Parkin interact to mediate mitophagy. Nat Neurosci. 16, 1257-65 (2013). 23933751

• Sample Type Confirmation:

  FBXO7 is supported by BioGPS gene expression data to be expressed in MCF7

• Protein Accession #: NP_036311
• Purification: Affinity Purified
• Nucleotide Accession #: NM_012179
• Tested Species Reactivity: Human
• Gene Symbol: FBXO7
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish
• Application: IP, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 83%; Horse: 92%; Human: 100%; Mouse: 92%; Pig: 93%; Rabbit: 100%; Rat: 93%; Zebrafish: 83%
• Image 1: Human Placenta, Human brain
  
  Host: Rabbit
  Target: FBXO7
  Positive control (+): Human Placenta (PL)
  Negative control (-): Human brain (BR)
  Antibody concentration: 1ug/ml
• Image 2: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.