Product Number |
ARP42737_P050 |
Product Page |
www.avivasysbio.com/acly-antibody-middle-region-arp42737-p050.html |
Name |
ACLY Antibody - middle region (ARP42737_P050) |
Protein Size (# AA) |
1101 amino acids |
Molecular Weight |
121kDa |
NCBI Gene Id |
47 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
ATP citrate lyase |
Description |
|
Alias Symbols |
ACL, ATPCL, CLATP |
Peptide Sequence |
Synthetic peptide located within the following region: LRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPA |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
0 |
Description of Target |
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acety |
Protein Interactions |
UBC; UBR4; SIRT2; HUWE1; ISG15; KAT2B; SUMO2; SUMO3; STAU1; SUMO1; NEDD8; MDM2; ASB15; WWOX; TRIM25; MAGED2; BAG3; PPP6C; NCL; MCM6; MCM2; KPNA4; KPNA2; PRMT1; CDKN2A; SRRT; UBD; FN1; APP; SHC1; CUL3; CAND1; CUL2; SIRT7; UL27; HDAC5; TERF2; TERF1; GSK3B; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-ACLY (ARP42737_P050) antibody |
Blocking Peptide |
For anti-ACLY (ARP42737_P050) antibody is Catalog # AAP42737 (Previous Catalog # AAPP24961) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human ACLY |
Uniprot ID |
P53396 |
Protein Name |
ATP-citrate synthase |
Publications |
Acetyl-CoA Metabolism Supports Multistep Pancreatic Tumorigenesis. Cancer Discov. 9, 416-435 (2019). 30626590
Infantino, V., Iacobazzi, V., Palmieri, F. & Menga, A. ATP-citrate lyase is essential for macrophage inflammatory response. Biochem. Biophys. Res. Commun. 440, 105-11 (2013). 24051091 |
Sample Type Confirmation |
ACLY is strongly supported by BioGPS gene expression data to be expressed in ACHN |
Protein Accession # |
NP_001087 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_001096 |
Tested Species Reactivity |
Human |
Gene Symbol |
ACLY |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Sheep, Zebrafish |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 86%; Rat: 100%; Sheep: 93%; Zebrafish: 86% |
Image 1 | Human liver tissue
| Rabbit Anti-ACLY Antibody Catalog Number: ARP42737_P050 Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
|
Image 2 | Human ACHN
| WB Suggested Anti-ACLY Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: ACHN cell lysateACLY is strongly supported by BioGPS gene expression data to be expressed in Human ACHN cells |
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