ACTN4 Antibody - N-terminal region (ARP42202_T100)

• Product Number: ARP42202_T100
• Name: ACTN4 Antibody - N-terminal region (ARP42202_T100)
• Protein Size (# AA): 911 amino acids
• Molecular Weight: 100kDa
• NCBI Gene Id: 81
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Actinin, alpha 4
• Description:
  View product citations for ACTN4 Antibody - N-terminal region (ARP42202_T100) on CiteAb
• Alias Symbols: FSGS, FSGS1, ACTININ-4
• Peptide Sequence: Synthetic peptide located within the following region: LIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kimura,K., (2006) Genome Res. 16 (1), 55-65
• Description of Target: Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN4 is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in its gene have been associated with focal and segmental glomerulosclerosis.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
• Protein Interactions: MYOZ2; HUWE1; UBC; BMP7; ACTN3; SUMO2; SUMO3; GNB2; SUMO1; NEDD8; MDM2; HGS; SF3A3; VCP; CDKN2A; ACTN1; PDLIM1; UBD; IGSF8; ICAM1; CD81; SVIL; Fcho2; POT1; VCAM1; TXN; RPS27; MAPK7; KPNA2; ITGA4; FN1; ESR1; CDH1; TRAF3IP1; FMNL1; NOS3; ACTN2; UBASH3B; SHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-ACTN4 (ARP42202_T100) antibody is Catalog # AAP42202 (Previous Catalog # AAPP24625)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN4
• Uniprot ID: O43707
• Protein Name: Alpha-actinin-4
• Publications:

  Wei, S., Gao, X., Du, J., Su, J. & Xu, Z. Angiogenin enhances cell migration by regulating stress fiber assembly and focal adhesion dynamics. PLoS One 6, e28797 (2011). 22194915

• Sample Type Confirmation:

  ACTN4 is supported by BioGPS gene expression data to be expressed in HCT116

• Protein Accession #: NP_004915
• Purification: Protein A purified
• Nucleotide Accession #: NM_004924
• Tested Species Reactivity: Human
• Gene Symbol: ACTN4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Image 1: Human HCT116
  
  WB Suggested Anti-ACTN4 Antibody Titration: 0.625ug/ml
  Positive Control: HCT116 cell lysateACTN4 is supported by BioGPS gene expression data to be expressed in HCT116
• Image 2: ACTNX-GFP transfected
  
  Lanes:
  Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate
  Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate
  Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate
  Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate
  Primary Antibody Dilution:
  1: 1000
  Secondary Antibody:
  Anti-rabbit HRP
  Secondary Antibody Dilution:
  1:5000
  Gene Name:
  ACTN4
  Submitted by:
  Johannes W. Hell, University of California
  
• Image 3: ACTNX-GFP transfected
  
  Sample Type:
  ACTNX-GFP transfected COS-7 cells
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit-Alexa-Fluor 568
  Secondary Antibody Dilution:
  1:100
  Color/Signal Descriptions:
  Green: GFP Red: ACTN4
  Gene Name:
  ACTN4
  Submitted by:
  Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710