Product Number |
ARP42065_P050 |
Product Page |
www.avivasysbio.com/map2k3-antibody-c-terminal-region-arp42065-p050.html |
Name |
MAP2K3 Antibody - C-terminal region (ARP42065_P050) |
Protein Size (# AA) |
318 amino acids |
Molecular Weight |
36kDa |
NCBI Gene Id |
5606 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Mitogen-activated protein kinase kinase 3 |
Description |
|
Alias Symbols |
MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2 |
Peptide Sequence |
Synthetic peptide located within the following region: EEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKK |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Prickett,T.D. (2007) Mol. Cell. Biol. 27 (12), 4217-4227 |
Description of Target |
MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. |
Protein Interactions |
UBC; EGFR; MAPK14; MAP3K5; ARRB1; PKN1; ALDOC; APP; LRRK2; MAPK8IP2; MAPK8IP3; MAPK8IP1; SPAG9; MAP3K2; MAP2K3; PPP2R4; MAP3K4; MAP3K3; TINF2; MYC; RPL13; TAOK2; TAOK1; DCTN1; DYRK1B; PLCB2; SMAD7; MAPK12; MAPK3; ELK1; MAP2K6; NPHS1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-MAP2K3 (ARP42065_P050) antibody |
Blocking Peptide |
For anti-MAP2K3 (ARP42065_P050) antibody is Catalog # AAP42065 (Previous Catalog # AAPP24544) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human MAP2K3 |
Uniprot ID |
P46734-2 |
Protein Name |
Dual specificity mitogen-activated protein kinase kinase 3 |
Sample Type Confirmation |
MAP2K3 is strongly supported by BioGPS gene expression data to be expressed in 721_B |
Protein Accession # |
NP_002747 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_002756 |
Tested Species Reactivity |
Human |
Gene Symbol |
MAP2K3 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 93%; Zebrafish: 100% |
Image 1 | Human Liver Tissue
| MAP2K3 antibody - C-terminal region (ARP42065_P050)
Catalog Number: ARP42065_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
Image 2 | Human 721_B
| WB Suggested Anti-MAP2K3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: 721_B cell lysateMAP2K3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells |
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