MAP2K3 Antibody - N-terminal region (ARP42064_P050)

Data Sheet
 
Product Number ARP42064_P050
Product Page www.avivasysbio.com/map2k3-antibody-n-terminal-region-arp42064-p050.html
Name MAP2K3 Antibody - N-terminal region (ARP42064_P050)
Protein Size (# AA) 318 amino acids
Molecular Weight 36kDa
NCBI Gene Id 5606
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Mitogen-activated protein kinase kinase 3
Alias Symbols MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2
Peptide Sequence Synthetic peptide located within the following region: SKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEK
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Prickett,T.D. (2007) Mol. Cell. Biol. 27 (12), 4217-4227
Description of Target MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Protein Interactions UBC; EGFR; MAPK14; MAP3K5; ARRB1; PKN1; ALDOC; APP; LRRK2; MAPK8IP2; MAPK8IP3; MAPK8IP1; SPAG9; MAP3K2; MAP2K3; PPP2R4; MAP3K4; MAP3K3; TINF2; MYC; RPL13; TAOK2; TAOK1; DCTN1; DYRK1B; PLCB2; SMAD7; MAPK12; MAPK3; ELK1; MAP2K6; NPHS1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-MAP2K3 (ARP42064_P050) antibody
Blocking Peptide For anti-MAP2K3 (ARP42064_P050) antibody is Catalog # AAP42064 (Previous Catalog # AAPP24543)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MAP2K3
Uniprot ID P46734-2
Protein Name Dual specificity mitogen-activated protein kinase kinase 3
Protein Accession # NP_002747
Purification Affinity Purified
Nucleotide Accession # NM_002756
Tested Species Reactivity Human
Gene Symbol MAP2K3
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 86%; Dog: 93%; Goat: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%
Image 1
Human Liver Tissue
MAP2K3 antibody - N-terminal region (ARP42064_P050)
Catalog Number: ARP42064_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 2
Transfected 293T
WB Suggested Anti-MAP2K3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T
 

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