Product Number |
ARP42029_P050 |
Product Page |
www.avivasysbio.com/krt8-antibody-middle-region-arp42029-p050.html |
Name |
KRT8 Antibody - middle region (ARP42029_P050) |
Protein Size (# AA) |
483 amino acids |
Molecular Weight |
54kDa |
NCBI Gene Id |
3856 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Keratin 8 |
Alias Symbols |
K8, KO, CK8, CK-8, CYK8, K2C8, CARD2 |
Peptide Sequence |
Synthetic peptide located within the following region: QSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Moon,M.C., (2008) Can. J. Physiol. Pharmacol. 86 (5), 223-231 |
Description of Target |
KRT8 together with KRT19, help to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
Protein Interactions |
KRT31; KRT15; KRT13; KRT40; TFIP11; KRT38; UBC; MDM2; ASB12; EED; GRB2; FBXO25; PAN2; IQCB1; IKBKG; VCAM1; NOS2; KRT18; KPNA2; ITGA4; FN1; ATF2; CDH1; STAM2; CRK; UBASH3B; APEX1; RCHY1; SQSTM1; SUMO1; SUMO2; SUMO3; MAPK8; QRSL1; YWHAQ; TJP1; TADA2A; USP32 |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-KRT8 (ARP42029_P050) antibody |
Blocking Peptide |
For anti-KRT8 (ARP42029_P050) antibody is Catalog # AAP42029 (Previous Catalog # AAPP24510) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human KRT8 |
Uniprot ID |
P05787 |
Protein Name |
Keratin, type II cytoskeletal 8 |
Sample Type Confirmation |
KRT8 is supported by BioGPS gene expression data to be expressed in HepG2, MCF7 |
Protein Accession # |
NP_002264 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_002273 |
Tested Species Reactivity |
Human |
Gene Symbol |
KRT8 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 86%; Rat: 93% |
Image 1 | Human Small intestine
| Immunohistochemistry with Small intestine tissue at an antibody concentration of 5ug/ml using anti-KRT8 antibody (ARP42029_P050) |
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Image 2 | Human Liver Tissue
| KRT8 antibody - middle region (ARP42029_P050)
Catalog Number: ARP42029_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm and membrane in epithelial cells of hepatic ducts
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
Image 3 | Human Adult Placenta
| Host: Rabbit Target Name: KRT8 Sample Type: Human Adult Placenta Antibody Dilution: 1.0ug/ml |
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Image 4 | Human Fetal Heart
| Host: Rabbit Target Name: KRT8 Sample Type: Human Fetal Heart Antibody Dilution: 1.0ug/ml |
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Image 5 | Human Fetal Lung
| Host: Rabbit Target Name: KRT8 Sample Type: Human Fetal Lung Antibody Dilution: 1.0ug/ml |
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Image 6 | Human MCF7
| Host: Rabbit Target Name: KRT8 Sample Type: Human MCF7 Antibody Dilution: 1.0ug/mlKRT8 is supported by BioGPS gene expression data to be expressed in MCF7 |
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Image 7 | Human HepG2
| WB Suggested Anti-KRT8 Antibody Titration: 0.2-1 ug/ml Positive Control: HepG2 cell lysateKRT8 is supported by BioGPS gene expression data to be expressed in HepG2 |
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