TSHR Antibody - C-terminal region (ARP41871_P050)

• Product Number: ARP41871_P050
• Name: TSHR Antibody - C-terminal region (ARP41871_P050)
• Protein Size (# AA): 764 amino acids
• Molecular Weight: 87 kDa
• NCBI Gene Id: 7253
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Thyroid stimulating hormone receptor
• Alias Symbols: LGR3, CHNG1, hTSHR-I
• Peptide Sequence: Synthetic peptide located within the following region: KLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLLPLGRKSLSFETQKAPRSS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Claus,M., (2005) Endocrinology 146 (12), 5197-5203
• Description of Target: TSHR is receptor for thyrothropin. It plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. It also acts as a receptor for thyrostimulin (GPA2+GPB5).
• Protein Interactions: ADRB2; SCRIB; UBC; TSHB; RABAC1; GNA13; GNA12; FLNB; TSHR; GNAQ; PIK3R1; LHCGR; JAK2; JAK1; GNAI3; GNAI2; GNAS; GNA11; GNAO1; GNAI1; HSPA5; FN1; CANX; CALR; STAT3; CALCR;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-TSHR (ARP41871_P050) antibody is Catalog # AAP41871 (Previous Catalog # AAPP24408)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TSHR
• Uniprot ID: Q96GT6
• Protein Name: Thyrotropin receptor
• Publications:

  Pessina, P., Castillo, V., Sartore, I., Borrego, J. & Meikle, A. Semiquantitative immunohistochemical marker staining and localization in canine thyroid carcinoma and normal thyroid gland. Vet. Comp. Oncol. (2014). doi:10.1111/vco.12111 25082554

• Sample Type Confirmation:

  There is BioGPS gene expression data showing that TSHR is expressed in Jurkat

• Protein Accession #: NP_001018046
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001018036
• Tested Species Reactivity: Human
• Gene Symbol: TSHR
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 83%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 86%; Rabbit: 86%; Rat: 93%; Sheep: 79%
• Image 1: Human Jurkat
  
  WB Suggested Anti-TSHR Antibody Titration: 0.2-1 ug/ml
  Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that TSHR is expressed in Jurkat. The peptide sequence is present in a 28 kDa isoform.
• Image 2: Human HepG2 Whole Cell
  
  Host: Rabbit
  Target Name: TSHR
  Sample Tissue: Human HepG2 Whole Cell
  Antibody Dilution: 1ug/ml