F2 Antibody - middle region (ARP41757_P050)

• Product Number: ARP41757_P050
• Name: F2 Antibody - middle region (ARP41757_P050)
• Protein Size (# AA): 622 amino acids
• Molecular Weight: 70 kDa
• NCBI Gene Id: 2147
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Coagulation factor II (thrombin)
• Description:
  View product citations for F2 Antibody - middle region (ARP41757_P050) on CiteAb
• Alias Symbols: PT, THPH1, RPRGL2
• Peptide Sequence: Synthetic peptide located within the following region: ISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Rossi,E., (2008) Thromb. Haemost. 99 (6), 1030-1034
• Description of Target: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life.Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: ST13; FN1; DDIT3; ALB; F11; PROZ; ITGA2B; SERPING1; HGFAC; AKR7A2; CPB2; F2RL3; F2RL2; SERPINA5; SERPINB8; AANAT; F2RL1; F9; F8; GP1BA; KNG1; F5; C6; PLAU; THBS1; THBD; F2R; SERPINE2; F2; PROS1; AMBP; PROC; GP5; SERPINE1; SERPINB6; SPP1; IGFBP5; IGFBP3; S
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Additional Information: IHC Information: Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
  IHC Information: Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
• Blocking Peptide: For anti-F2 (ARP41757_P050) antibody is Catalog # AAP41757 (Previous Catalog # AAPP10805)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human F2
• Uniprot ID: P00734
• Protein Name: Prothrombin
• Protein Accession #: NP_000497
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000506
• Tested Species Reactivity: Human
• Gene Symbol: F2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Sheep, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Sheep: 93%; Zebrafish: 77%
• Image 1: Human 721_B
  
  WB Suggested Anti-F2 Antibody
  Titration: 1 ug/ml
  Positive Control: 721_B cell lysate
• Image 2: Human Liver
  
  Anti-F2 / Thrombin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
• Image 3: Human Placenta
  
  Anti-F2 / Thrombin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
• Image 4: Human Placenta
  
  Rabbit Anti-F2 Antibody
  Catalog Number: arp41757
  Paraffin Embedded Tissue: Human Placenta
  Antibody Concentration: 5 ug/ml
• Image 5: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Protein is processed to prothrombin and the peptide also is contained in the b-chain form of ~30 kDa as well as a 32 kDa form of prothrombin. Peptide aligns to amino acids 419-432 kDa in the full length form.