ALB Antibody - N-terminal region (ARP41745_P050)

• Product Number: ARP41745_P050
• Name: ALB Antibody - N-terminal region (ARP41745_P050)
• Protein Size (# AA): 609 amino acids
• Molecular Weight: 66kDa
• NCBI Gene Id: 213
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Albumin
• Alias Symbols: HSA, PRO0883, PRO0903, PRO1341
• Peptide Sequence: Synthetic peptide located within the following region: YGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEET
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Amirtharaj,G.J., (2008) Biochim. Biophys. Acta 1782 (5), 349-354
• Description of Target: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: FUS; UBC; PARK2; UBD; PAN2; MYLK3; CABLES1; SLA2; TLN2; CEP44; PHC3; PALB2; QTRTD1; TTPAL; DERL1; CDCP1; TRAPPC11; SCAF1; NLRC4; IGDCC4; GJC2; CROT; EXOC6; PDZRN4; EEF2K; IGKV2-40; BBC3; LAT; SACS; SPATA31A7; AP4E1; CRB1; DICER1; OBSL1; SLC9A8; CAMTA1; SE
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: Paraffin embedded kidney tissue, tested with an antibody dilution of 5 ug/ml.
• Blocking Peptide: For anti-ALB (ARP41745_P050) antibody is Catalog # AAP41745 (Previous Catalog # AAPP24387)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ALB
• Uniprot ID: P02768
• Protein Name: Serum albumin
• Protein Accession #: NP_000468
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000477
• Tested Species Reactivity: Human
• Gene Symbol: ALB
• Predicted Species Reactivity: Human, Mouse, Rat, Guinea Pig, Horse
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Guinea Pig: 100%; Horse: 82%; Human: 100%; Mouse: 90%; Rat: 100%
• Image 1: Human Kidney
  
  Kidney
• Image 2:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.