ACTN2 Antibody - C-terminal region (ARP41463_T100)

Data Sheet
 
Product Number ARP41463_T100
Product Page www.avivasysbio.com/actn2-antibody-c-terminal-region-arp41463-t100.html
Name ACTN2 Antibody - C-terminal region (ARP41463_T100)
Protein Size (# AA) 894 amino acids
Molecular Weight 98kDa
NCBI Gene Id 88
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Actinin, alpha 2
Alias Symbols MPD6, CMH23, CMD1AA, MYOCOZ
Peptide Sequence Synthetic peptide located within the following region: VIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALD
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Eldstrom,J., FEBS Lett. 547 (1-3), 205-211 (2003)
Description of Target The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Transcript variants resulting from the use of multiple poly_A sites have been observed.
Protein Interactions ACTN2; FAM154A; BRMS1L; MICALL2; MYOZ2; PDLIM3; SP100; SNAI1; MOS; RTP5; SYNPO2; SUMO1; IGSF8; ERBB2IP; CAPN1; ZNF446; RPL35; UBC; NOS3; ACTN1; ACTN4; FBXL22; COIL; SUMO3; ATXN2; KCNA5; LDB3; KCNN2; PSMA3; ITGB3BP; SNW1; MED14; NR1I2; KAT2B; NRIP1; ATXN7;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ACTN2 (ARP41463_T100) antibody
Blocking Peptide For anti-ACTN2 (ARP41463_T100) antibody is Catalog # AAP41463 (Previous Catalog # AAPS09209)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human ACTN2
Uniprot ID P35609
Protein Name Alpha-actinin-2
Protein Accession # NP_001094
Purification Protein A purified
Nucleotide Accession # NM_001103
Tested Species Reactivity Human
Gene Symbol ACTN2
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
Image 1
Human PANC1
Host: Rabbit
Target Name: ACTN2
Sample Tissue: Human PANC1
Antibody Dilution: 1.0ug/ml
Image 2
ACTNX-GFP transfected
Lanes:
Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate
Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate
Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate
Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate
Primary Antibody Dilution:
1: 1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
ACTN2
Submitted by:
Johannes W. Hell, University of California
Image 3
Human Muscle
Rabbit Anti-ACTN2 Antibody
Catalog Number: ARP41463
Paraffin Embedded Tissue: Human Muscle
Cellular Data: Skeletal muscle cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
 

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