Product Number |
ARP41463_T100 |
Product Page |
www.avivasysbio.com/actn2-antibody-c-terminal-region-arp41463-t100.html |
Name |
ACTN2 Antibody - C-terminal region (ARP41463_T100) |
Protein Size (# AA) |
894 amino acids |
Molecular Weight |
98kDa |
NCBI Gene Id |
88 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Actinin, alpha 2 |
Alias Symbols |
MPD6, CMH23, CMD1AA, MYOCOZ |
Peptide Sequence |
Synthetic peptide located within the following region: VIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALD |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Eldstrom,J., FEBS Lett. 547 (1-3), 205-211 (2003) |
Description of Target |
The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Transcript variants resulting from the use of multiple poly_A sites have been observed. |
Protein Interactions |
ACTN2; FAM154A; BRMS1L; MICALL2; MYOZ2; PDLIM3; SP100; SNAI1; MOS; RTP5; SYNPO2; SUMO1; IGSF8; ERBB2IP; CAPN1; ZNF446; RPL35; UBC; NOS3; ACTN1; ACTN4; FBXL22; COIL; SUMO3; ATXN2; KCNA5; LDB3; KCNN2; PSMA3; ITGB3BP; SNW1; MED14; NR1I2; KAT2B; NRIP1; ATXN7; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-ACTN2 (ARP41463_T100) antibody |
Blocking Peptide |
For anti-ACTN2 (ARP41463_T100) antibody is Catalog # AAP41463 (Previous Catalog # AAPS09209) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human ACTN2 |
Uniprot ID |
P35609 |
Protein Name |
Alpha-actinin-2 |
Protein Accession # |
NP_001094 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_001103 |
Tested Species Reactivity |
Human |
Gene Symbol |
ACTN2 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86% |
Image 1 | Human PANC1
| Host: Rabbit Target Name: ACTN2 Sample Tissue: Human PANC1 Antibody Dilution: 1.0ug/ml |
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Image 2 | ACTNX-GFP transfected
| Lanes: Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate Primary Antibody Dilution: 1: 1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: ACTN2 Submitted by: Johannes W. Hell, University of California
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Image 3 | Human Muscle
| Rabbit Anti-ACTN2 Antibody Catalog Number: ARP41463 Paraffin Embedded Tissue: Human Muscle Cellular Data: Skeletal muscle cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
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