ACTN2 Antibody - N-terminal region : HRP (ARP41462_P050-HRP)

• Product Number: ARP41462_P050-HRP
• Name: ACTN2 Antibody - N-terminal region : HRP (ARP41462_P050-HRP)
• Protein Size (# AA): 894 amino acids
• Molecular Weight: 104kDa
• Conjugation: HRP: Horseradish Peroxidase
• NCBI Gene Id: 88
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Actinin, alpha 2
• Alias Symbols: MPD6, CMH23, CMD1AA, MYOCOZ
• Peptide Sequence: Synthetic peptide located within the following region: NQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSH
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Reference: Bos,J.M., (2008) Am. Heart J. 155 (6), 1128-1134
• Description of Target: The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-39 DC378038.1 17-55 40-2961 BC051770.1 1-2922 2962-3458 CB153006.1 65-561 3459-3637 AL359185.25 28531-28709 3638-4210 M86406.1 3609-4181 4211-4528 AL359185.25 29283-29600
• Protein Interactions: ACTN2; FAM154A; BRMS1L; MICALL2; MYOZ2; PDLIM3; SP100; SNAI1; MOS; RTP5; SYNPO2; SUMO1; IGSF8; ERBB2IP; CAPN1; ZNF446; RPL35; UBC; NOS3; ACTN1; ACTN4; FBXL22; COIL; SUMO3; ATXN2; KCNA5; LDB3; KCNN2; PSMA3; ITGB3BP; SNW1; MED14; NR1I2; KAT2B; NRIP1; ATXN7;
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Blocking Peptide: For anti-ACTN2 (ARP41462_P050-HRP) antibody is Catalog # AAP41462 (Previous Catalog # AAPS09208)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN2
• Uniprot ID: P35609
• Protein Name: Alpha-actinin-2
• Publications:

  Yan, Z. et al. BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse embryonic stem cells. Stem Cells 26, 1155-65 (2008). ICC/IF, Human, Mouse, Rat, Rabbit, Guinea pig, Dog, Horse, Bovine 18323406

• Protein Accession #: NP_001094
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001103
• Gene Symbol: ACTN2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 93%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: