ACTN2 Antibody - N-terminal region (ARP41462_P050)

• Product Number: ARP41462_P050
• Name: ACTN2 Antibody - N-terminal region (ARP41462_P050)
• Protein Size (# AA): 894 amino acids
• Molecular Weight: 104 kDa
• NCBI Gene Id: 88
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Actinin, alpha 2
• Alias Symbols: MPD6, CMH23, CMD1AA, MYOCOZ
• Peptide Sequence: Synthetic peptide located within the following region: NQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSH
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Bos,J.M., (2008) Am. Heart J. 155 (6), 1128-1134
• Description of Target: The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-39 DC378038.1 17-55 40-2961 BC051770.1 1-2922 2962-3458 CB153006.1 65-561 3459-3637 AL359185.25 28531-28709 3638-4210 M86406.1 3609-4181 4211-4528 AL359185.25 29283-29600
• Protein Interactions: ACTN2; FAM154A; BRMS1L; MICALL2; MYOZ2; PDLIM3; SP100; SNAI1; MOS; RTP5; SYNPO2; SUMO1; IGSF8; ERBB2IP; CAPN1; ZNF446; RPL35; UBC; NOS3; ACTN1; ACTN4; FBXL22; COIL; SUMO3; ATXN2; KCNA5; LDB3; KCNN2; PSMA3; ITGB3BP; SNW1; MED14; NR1I2; KAT2B; NRIP1; ATXN7;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-ACTN2 (ARP41462_P050) antibody is Catalog # AAP41462 (Previous Catalog # AAPS09208)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN2
• Uniprot ID: P35609
• Protein Name: Alpha-actinin-2
• Publications:

  Yan, Z. et al. BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse embryonic stem cells. Stem Cells 26, 1155-65 (2008). 18323406

• Protein Accession #: NP_001094
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001103
• Tested Species Reactivity: Human
• Gene Symbol: ACTN2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 93%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: ACTNX-GFP transfected
  
  Lanes:
  Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate
  Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate
  Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate
  Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate
  Primary Antibody Dilution:
  1: 1000
  Secondary Antibody:
  Anti-rabbit HRP
  Secondary Antibody Dilution:
  1:5000
  Gene Name:
  ACTN2
  Submitted by:
  Johannes W. Hell, University of California
  
• Image 2: ACTNX-GFP transfected
  
  Sample Type:
  ACTNX-GFP transfected COS-7 cells
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit-Alexa-Fluor 568
  Secondary Antibody Dilution:
  1:100
  Color/Signal Descriptions:
  Green: GFP Red: ACTN2
  Gene Name:
  ACTN2
  Submitted by:
  Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710
• Image 3: Human Muscle
  
  WB Suggested Anti-ACTN2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: Human Muscle
• Image 4: Human Heart
  
  Rabbit Anti-ACTN2 antibody
  Catalog Number: ARP41462
  Formalin Fixed Paraffin Embedded Tissue: Human Adult Heart
  Observed Staining: Cytoplasm in hepatocytes
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec