TPM1 Antibody - N-terminal region (ARP41392_T100)

• Product Number: ARP41392_T100
• Name: TPM1 Antibody - N-terminal region (ARP41392_T100)
• Protein Size (# AA): 284 amino acids
• Molecular Weight: 31kDa
• NCBI Gene Id: 7168
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Tropomyosin 1 (alpha)
• Alias Symbols: CMH3, TMSA, CMD1Y, LVNC9, C15orf13, HEL-S-265, HTM-alpha
• Peptide Sequence: Synthetic peptide located within the following region: DRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Lin,J.C. (2005) Mol. Cell. Biol. 25 (22), 10111-10121
• Description of Target: TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction.This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.
• Protein Interactions: GOLGA2; CAGE1; C1orf216; SYCE1; KXD1; TFPT; MAD1L1; TPM4; TP53; TNNT1; UBC; MDM2; ETFA; FOXK1; KIAA1598; PPP6R3; APPL2; CDC37; MGEA5; STUB1; ABI2; DNAJC7; HSP90B1; PARK2; LRCH3; PAN2; TGFBR1; SPTBN1; ATF2; MDC1; BRCA1; PPP4R2; SRXN1; NUDCD2; RBM15; THOC2;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TPM1 (ARP41392_T100) antibody is Catalog # AAP41392 (Previous Catalog # AAPP24130)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TPM1
• Uniprot ID: P09493
• Protein Name: Tropomyosin alpha-1 chain
• Protein Accession #: NP_000357
• Purification: Protein A purified
• Nucleotide Accession #: NM_000366
• Tested Species Reactivity: Human
• Gene Symbol: TPM1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 93%; Zebrafish: 100%
• Image 1: Stomach tumor, 293T
  
  Host: Rabbit
  Target: TPM1
  Positive control (+): Stomach tumor (T-ST)
  Negative control (-): 293T (2T)
  Antibody concentration: 1ug/ml
• Image 2: Human Hela
  
  Host: Rabbit
  Target Name: TPM1
  Sample Tissue: Human Hela
  Antibody Dilution: 1.0ug/ml
• Image 3: Human Muscle
  
  Rabbit Anti-TPM1 Antibody
  Catalog Number: ARP41392
  Paraffin Embedded Tissue: Human Muscle
  Cellular Data: Skeletal muscle cells
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X