EIF2A Antibody - C-terminal region (ARP41041_T100)

• Product Number: ARP41041_T100
• Name: EIF2A Antibody - C-terminal region (ARP41041_T100)
• Protein Size (# AA): 585 amino acids
• Molecular Weight: 65 kDa
• NCBI Gene Id: 83939
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Eukaryotic translation initiation factor 2A, 65kDa
• Alias Symbols: CDA02, EIF-2A, MST089, MSTP004, MSTP089
• Peptide Sequence: Synthetic peptide located within the following region: DKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQ
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Su,Q., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (1), 63-68
• Description of Target: EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes.EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002).[supplied by OMIM].
• Protein Interactions: UBC; CDC20; RPS23; RPS19; RPS16; RPS10; RPS7; RPS5; HDLBP; PRUNE2; EIF3K; SERBP1; RPS29; RPS24; EIF2AK2; MLH1; NXF1; ILF3; PA2G4; APP; PPP1CC; NEDD8; DCC; ATF4;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-EIF2A (ARP41041_T100) antibody is Catalog # AAP41041 (Previous Catalog # AAPS02304)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EIF2A
• Uniprot ID: Q9BY44
• Protein Name: Eukaryotic translation initiation factor 2A
• Publications:

  Xu, Y.-X., Liu, L., Caffaro, C. E. & Hirschberg, C. B. Inhibition of Golgi apparatus glycosylation causes endoplasmic reticulum stress and decreased protein synthesis. J. Biol. Chem. 285, 24600-8 (2010). 20529871

• Protein Accession #: NP_114414
• Purification: Protein A purified
• Nucleotide Accession #: NM_032025
• Tested Species Reactivity: Human
• Gene Symbol: EIF2A
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 100%; Goat: 83%; Guinea Pig: 93%; Horse: 86%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%
• Image 1: Human Skin
  
  Rabbit Anti-EIF2A Antibody
  Catalog Number: ARP41041
  Paraffin Embedded Tissue: Human Skin
  Cellular Data: Epidermal cells
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2:
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The peptide is also present in a 62 kDa isoform. The protein may be modified by phosphorylation.