CPEB4 Antibody - N-terminal region (ARP41024_P050)

• Product Number: ARP41024_P050
• Name: CPEB4 Antibody - N-terminal region (ARP41024_P050)
• Protein Size (# AA): 729 amino acids
• Molecular Weight: 80 kDa
• NCBI Gene Id: 80315
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Cytoplasmic polyadenylation element binding protein 4
• Description:
  View product citations for CPEB4 Antibody - N-terminal region (ARP41024_P050) on CiteAb
• Alias Symbols: CPE-BP4, hCPEB-4
• Peptide Sequence: Synthetic peptide located within the following region: DEILGSEKAKSQQQEQQDPLEKQQLSPSPGQEAGILPETEKAKSEENQGD
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kurihara,Y., (2003) Biol. Reprod. 69 (1), 261-268
• Description of Target: The exact function of CPEB4 remains unknown.
• Protein Interactions: BMI1; SAV1; UBE3C; NDST1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-CPEB4 (ARP41024_P050) antibody is Catalog # AAP41024 (Previous Catalog # AAPS02204)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CPEB4
• Uniprot ID: Q17RY0
• Protein Name: Cytoplasmic polyadenylation element-binding protein 4
• Publications:

  Autism-like phenotype and risk gene mRNA deadenylation by CPEB4 mis-splicing. Nature. 560, 441-446 (2018). 30111840

• Protein Accession #: NP_085130
• Purification: Affinity Purified
• Nucleotide Accession #: NM_030627
• Tested Species Reactivity: Human
• Gene Symbol: CPEB4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 83%
• Image 1: Human Jurkat Whole Cell
  
  Host: Rabbit
  Target Name: CPEB4
  Sample Tissue: Human Jurkat Whole Cell
  Antibody Dilution: 1ug/ml
• Image 2: Human Lung
  
  WB Suggested Anti-CPEB4 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: Human Lung
• Image 3: Human DLD1 Whole Cell
  
  Host: Rabbit
  Target Name: CPEB4
  Sample Tissue: Human DLD1 Whole Cell
  Antibody Dilution: 1ug/ml
• Image 4:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.