Product Number |
ARP40715_T100 |
Product Page |
www.avivasysbio.com/nono-antibody-n-terminal-region-arp40715-t100.html |
Name |
NONO Antibody - N-terminal region (ARP40715_T100) |
Protein Size (# AA) |
471 amino acids |
Molecular Weight |
52kDa |
NCBI Gene Id |
4841 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Non-POU domain containing, octamer-binding |
Alias Symbols |
P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114 |
Peptide Sequence |
Synthetic peptide located within the following region: KQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLK |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Kimura,K., (2006) Genome Res. 16 (1), 55-65 |
Description of Target |
NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. |
Protein Interactions |
SFPQ; PTBP1; PRKAA2; PIN1; NONO; FUS; DDX6; C11orf68; PSPC1; HUWE1; LMO4; UBC; SUMO2; STAU1; MDM2; RPA3; RPA2; RPA1; ASB18; WWOX; ASB3; ERG; EED; rev; WBP4; TCERG1; APBB1; PRPF40A; LMNA; CD81; PAN2; CTNNB1; ORC5; MYC; ITGA4; IL7R; FN1; EWSR1; YLPM1; RNF43 |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-NONO (ARP40715_T100) antibody |
Blocking Peptide |
For anti-NONO (ARP40715_T100) antibody is Catalog # AAP40715 (Previous Catalog # AAPY00967) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human NONO |
Uniprot ID |
Q15233 |
Protein Name |
Non-POU domain-containing octamer-binding protein |
Publications |
Bubenik, J. L., Ladd, A. N., Gerber, C. A., Budiman, M. E. & Driscoll, D. M. Known turnover and translation regulatory RNA-binding proteins interact with the 3â UTR of SECIS-binding protein 2. RNA Biol. 6, 73-83 (2009). 19106619 |
Protein Accession # |
NP_031389 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_007363 |
Tested Species Reactivity |
Human |
Gene Symbol |
NONO |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse |
Application |
IF, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100% |
Image 1 | subnuclear bodies
| Sample Type : subnuclear bodies-paraspeckles
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Image 2 | Human HepG2
| WB Suggested Anti-NONO Antibody Titration: 1.25ug/ml ELISA Titer: 1:312500 Positive Control: HepG2 cell lysate |
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