| Product Number |
ARP40417_P050 |
| Product Page |
www.avivasysbio.com/psma1-antibody-c-terminal-region-arp40417-p050.html |
| Name |
PSMA1 Antibody - C-terminal region (ARP40417_P050) |
| Protein Size (# AA) |
263 amino acids |
| Molecular Weight |
29kDa |
| Subunit |
alpha type-1 |
| NCBI Gene Id |
5682 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Proteasome (prosome, macropain) subunit, alpha type, 1 |
| Alias Symbols |
NU, HC2, PROS30, HEL-S-275 |
| Peptide Sequence |
Synthetic peptide located within the following region: TYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDL |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Conticello,S.G., (2003) Curr. Biol. 13 (22), 2009-2013 |
| Description of Target |
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA1 is a member of the peptidase T1A family which is a 20S core alpha subunit.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. |
| Protein Interactions |
PRDM14; CEP70; CCDC102B; GNPTAB; CEP72; KCTD9; ROPN1; ABI3; SH3GLB1; APIP; LDOC1; MTUS2; MAPRE3; MAPRE1; IKZF3; MID2; EHMT2; PNMA2; IKZF1; TRIM10; HUWE1; HOMER3; PNMA1; KRT38; BLZF1; MAD1L1; MKRN3; REL; PSMA3; MLH1; KRT31; KRT15; KRTAP5-9; GOLGA2; CDA; CC |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PSMA1 (ARP40417_P050) antibody |
| Blocking Peptide |
For anti-PSMA1 (ARP40417_P050) antibody is Catalog # AAP40417 (Previous Catalog # AAPP23493) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human PSMA1 |
| Uniprot ID |
P25786 |
| Protein Name |
Proteasome subunit alpha type-1 |
| Publications |
Cron, K. R. et al. Proteasome inhibitors block DNA repair and radiosensitize non-small cell lung cancer. PLoS One 8, e73710 (2013). 24040035 |
| Sample Type Confirmation |
PSMA1 is strongly supported by BioGPS gene expression data to be expressed in NCI460 |
| Protein Accession # |
NP_002777 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_002786 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
PSMA1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-PSMA1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysate |
|
| Image 2 | Human NCI460
 | Sample Type: Human non-small cell lung cancer (NCI-460)Primary Dilution: 1:2000Secondary Dilution: 1:300050kDa band is a tubulin loading control bandPSMA1 is strongly supported by BioGPS gene expression data to be expressed in Human NCI460 cells |
|
| Image 3 | Human Bronchial Epithelial Tissue
 | Rabbit Anti-PSMA1 Antibody
Catalog Number: ARP40417_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
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