HNRPA1 Antibody - N-terminal region : FITC (ARP40383_T100-FITC)

Data Sheet
 
Product Number ARP40383_T100-FITC
Product Page www.avivasysbio.com/hnrpa1-antibody-n-terminal-region-fitc-arp40383-t100-fitc.html
Name HNRPA1 Antibody - N-terminal region : FITC (ARP40383_T100-FITC)
Protein Size (# AA) 320 amino acids
Molecular Weight 35kDa
Conjugation FITC: Fluorescein Isothiocyanate
NCBI Gene Id 3178
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Heterogeneous nuclear ribonucleoprotein A1
Alias Symbols UP 1, ALS19, ALS20, HNRPA1, IBMPFD3, HNRPA1L3, hnRNP A1, hnRNP-A1
Peptide Sequence Synthetic peptide located within the following region: MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPN
Product Format Liquid. Purified antibody supplied in 1x PBS buffer.
Reference Tevosian,S.G., (2005) J. Biol. Chem. 280 (13), 13171-13178
Description of Target HNRPA1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). HNRPA1 has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. HNRPA1 is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites.
Protein Interactions UBC; TP53; PSMD9; TUBGCP3; NEDD1; AURKB; AURKA; STAU1; IVNS1ABP; TNF; PSMB4; MDM2; CHUK; RPA3; RPA2; RPA1; ASB2; ERG; EED; RNF2; rev; IQGAP1; HIST1H2AB; AIMP2; RPL38; RPL27A; QARS; DHX9; CCAR2; ZC3HC1; HNRNPU; PARK2; FBXO6; TARDBP; YWHAQ; SRPK1; XRN1; CSF
Reconstitution and Storage All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Datasheets/Manuals Printable datasheet for anti-HNRNPA1 (ARP40383_T100-FITC) antibody
Blocking Peptide For anti-HNRNPA1 (ARP40383_T100-FITC) antibody is Catalog # AAP40383 (Previous Catalog # AAPP22127)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HNRPA1
Uniprot ID Q6IPF2
Protein Name Heterogeneous nuclear ribonucleoprotein A1 EMBL AAH71945.1
Publications

Li, S. et al. Identification of an aptamer targeting hnRNP A1 by tissue slide-based SELEX. J. Pathol. 218, 327-36 (2009). ICC/IF, Horse, Human, Rat, Dog, Pig, Rabbit, Guinea pig, Mouse, Bovine 19291713

Gonen, N. & Assaraf, Y. G. The obligatory intestinal folate transporter PCFT (SLC46A1) is regulated by nuclear respiratory factor 1. J. Biol. Chem. 285, 33602-13 (2010). WB, Horse, Human, Rat, Dog, Pig, Rabbit, Guinea pig, Mouse, Bovine 20724482

Stark, M., Bram, E. E., Akerman, M., Mandel-Gutfreund, Y. & Assaraf, Y. G. Heterogeneous nuclear ribonucleoprotein H1/H2-dependent unsplicing of thymidine phosphorylase results in anticancer drug resistance. J. Biol. Chem. 286, 3741-54 (2011). WB, ICC/IF, ChIP, Horse, Human, Rat, Dog, Pig, Rabbit, Guinea pig, Mouse, Bovine 21068389

Li, S. et al. Aptamer BC15 against heterogeneous nuclear ribonucleoprotein A1 has potential value in diagnosis and therapy of hepatocarcinoma. Nucleic Acid Ther. 22, 391-8 (2012). WB, IHC, Horse, Human, Rat, Dog, Pig, Rabbit, Guinea pig, Mouse, Bovine 23062008

Sample Type Confirmation

HNRNPA1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_002127
Nucleotide Accession # NM_002136
Gene Symbol HNRNPA1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
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