AARS Antibody - C-terminal region (ARP40369_P050)

• Product Number: ARP40369_P050
• Name: AARS Antibody - C-terminal region (ARP40369_P050)
• Protein Size (# AA): 968 amino acids
• Molecular Weight: 107kDa
• NCBI Gene Id: 16
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Alanyl-tRNA synthetase
• Alias Symbols: AARS, CMT2N, DEE29, EIEE29
• Peptide Sequence: Synthetic peptide located within the following region: VTGAEAQKALRKAESLKKCLSVMEAKVKAQTAPNKDVQREIADLGEALAT
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Olsen,J.V., (2006) Cell 127 (3), 635-648
• Description of Target: The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
• Protein Interactions: ISG15; UBC; SUMO2; SUMO1; NEDD8; TPD52; STAT5B; SRP9; PPP2R5E; METTL1; HSF1; CALU; DPP9; NIF3L1; ANKMY2; DPP8; IPO11; EEF2K; CACYBP; ARPC1B; SAE1; TOM1L1; EIF4A3; WARS; TPD52L2; UBD; VCAM1; ITGA4; HSP90AB1; HSP90AA1; FN1; NSUN2; LARS; FARSB; UBA1; QARS; N
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-AARS (ARP40369_P050) antibody is Catalog # AAP40369 (Previous Catalog # AAPP22113)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AARS
• Uniprot ID: P49588
• Protein Name: Alanine--tRNA ligase, cytoplasmic
• Sample Type Confirmation:

  AARS is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_001596
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001605
• Tested Species Reactivity: Human
• Gene Symbol: AARS
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 79%
• Image 1: Human Pineal Tissue
  
  Rabbit Anti-AARS Antibody
  Catalog Number: ARP40369_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
  Observed Staining: Cytoplasmic in cell processes of pinealocytes
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 2: Human Jurkat
  
  WB Suggested Anti-AARS Antibody Titration: 0.2-1 ug/ml
  Positive Control: Jurkat cell lysateAARS is supported by BioGPS gene expression data to be expressed in Jurkat
• Image 3: Human Jurkat
  
  Host: Rabbit
  Target Name: AARS
  Sample Type: Jurkat
  Antibody Dilution: 1.0ug/mlAARS is supported by BioGPS gene expression data to be expressed in Jurkat
• Image 4: heart
  
  Rabbit Anti-AARS Antibody
  Catalog Number: ARP40369_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
  Observed Staining: Cytoplasmic
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy2/3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec
  Protocol located in Reviews and Data.