GAPDH Antibody - N-terminal region (ARP40175_P050)

Data Sheet
 
Product Number ARP40175_P050
Product Page www.avivasysbio.com/gapdh-antibody-n-terminal-region-arp40175-p050.html
Name GAPDH Antibody - N-terminal region (ARP40175_P050)
Protein Size (# AA) 335 amino acids
Molecular Weight 36 kDa
NCBI Gene Id 2597
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Glyceraldehyde-3-phosphate dehydrogenase
Alias Symbols G3PD, GAPD, HEL-S-162eP
Peptide Sequence Synthetic peptide located within the following region: IDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKW
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Rinne,T., (2008) Hum. Mol. Genet. 17 (13), 1968-1977
Description of Target GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Interactions FUS; HUWE1; UBC; SUMO2; SUMO3; STAU1; MDM2; GAPDH; ASB15; ASB16; RPA3; RPA2; RPA1; EED; SNCA; rev; HSPA9; HSPA1L; GAS7; TUBA1A; TUFM; RAP1GDS1; PPP5C; PPIB; YWHAG; TUBB; TUBA1C; PRPF40A; UBQLN1; DCPS; MAT2B; ACOT7; METAP2; FERMT2; GBP1; EEF1A1; TYMP; DFFA
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-GAPDH (ARP40175_P050) antibody
Blocking Peptide For anti-GAPDH (ARP40175_P050) antibody is Catalog # AAP40175 (Previous Catalog # AAPP22021)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH
Uniprot ID Q2TSD0
Protein Name Glyceraldehyde-3-phosphate dehydrogenase RuleBase RU003389
Publications

Fu, Y. et al. ABCA12 Regulates ABCA1-Dependent Cholesterol Efflux from Macrophages and the Development of Atherosclerosis. Cell Metab. 18, 225-38 (2013). 23931754

Kalinec, G. M. et al. Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways. Hear. Res. 313, 26-37 (2014). 24793116

Sample Type Confirmation

GAPDH is strongly supported by BioGPS gene expression data to be expressed in HeLa

Protein Accession # NP_002037
Purification Affinity Purified
Nucleotide Accession # NM_002046
Tested Species Reactivity Human, Mouse
Gene Symbol GAPDH
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Sheep: 100%
Image 1
293T
Host: Rabbit
Target Name: GAPDH
Sample Type: 293T
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12
Image 2
Human brain
WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human brain
Image 3
Mouse NIH-3T3
Lanes:
1. 50 ug NIH3T3 cytosolic extract 2. 50 ug NIH3T3 cytosolic extract 3. 50 ug NIH3T3 cytosolic extract 4. 50 ug NIH3T3 cytosolic extract
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:30000
Gene Name:
GAPDH
Submitted by:
Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania
Image 4
Human Raji
WB Suggested Anti-GAPDH antibody Titration: 1 ug/mL
Sample Type: Human Raji
Image 5
Human Heart Muscle
Rabbit Anti-GAPDH Antibody
Catalog Number: ARP40175_P050
Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 6
Human liver tissue
Rabbit Anti-GAPDH Antibody
Catalog Number: ARP40175_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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