Product Number |
ARP40175_P050 |
Product Page |
www.avivasysbio.com/gapdh-antibody-n-terminal-region-arp40175-p050.html |
Name |
GAPDH Antibody - N-terminal region (ARP40175_P050) |
Protein Size (# AA) |
335 amino acids |
Molecular Weight |
36 kDa |
NCBI Gene Id |
2597 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Glyceraldehyde-3-phosphate dehydrogenase |
Alias Symbols |
G3PD, GAPD, HEL-S-162eP |
Peptide Sequence |
Synthetic peptide located within the following region: IDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKW |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Rinne,T., (2008) Hum. Mol. Genet. 17 (13), 1968-1977 |
Description of Target |
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Protein Interactions |
FUS; HUWE1; UBC; SUMO2; SUMO3; STAU1; MDM2; GAPDH; ASB15; ASB16; RPA3; RPA2; RPA1; EED; SNCA; rev; HSPA9; HSPA1L; GAS7; TUBA1A; TUFM; RAP1GDS1; PPP5C; PPIB; YWHAG; TUBB; TUBA1C; PRPF40A; UBQLN1; DCPS; MAT2B; ACOT7; METAP2; FERMT2; GBP1; EEF1A1; TYMP; DFFA |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
Relative Expression (Western Blot) |
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Datasheets/Manuals |
Printable datasheet for anti-GAPDH (ARP40175_P050) antibody |
Blocking Peptide |
For anti-GAPDH (ARP40175_P050) antibody is Catalog # AAP40175 (Previous Catalog # AAPP22021) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH |
Uniprot ID |
Q2TSD0 |
Protein Name |
Glyceraldehyde-3-phosphate dehydrogenase RuleBase RU003389 |
Publications |
Fu, Y. et al. ABCA12 Regulates ABCA1-Dependent Cholesterol Efflux from Macrophages and the Development of Atherosclerosis. Cell Metab. 18, 225-38 (2013). 23931754
Kalinec, G. M. et al. Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways. Hear. Res. 313, 26-37 (2014). 24793116 |
Sample Type Confirmation |
GAPDH is strongly supported by BioGPS gene expression data to be expressed in HeLa |
Protein Accession # |
NP_002037 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_002046 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
GAPDH |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Sheep: 100% |
Image 1 | 293T
| Host: Rabbit Target Name: GAPDH Sample Type: 293T Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/lane Gel Concentration: 0.12 |
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Image 2 | Human brain
| WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Human brain |
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Image 3 | Mouse NIH-3T3
| Lanes: 1. 50 ug NIH3T3 cytosolic extract 2. 50 ug NIH3T3 cytosolic extract 3. 50 ug NIH3T3 cytosolic extract 4. 50 ug NIH3T3 cytosolic extract Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-Rabbit HRP Secondary Antibody Dilution: 1:30000 Gene Name: GAPDH Submitted by: Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania
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Image 4 | Human Raji
| WB Suggested Anti-GAPDH antibody Titration: 1 ug/mL Sample Type: Human Raji |
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Image 5 | Human Heart Muscle
| Rabbit Anti-GAPDH Antibody Catalog Number: ARP40175_P050 Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:600 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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Image 6 | Human liver tissue
| Rabbit Anti-GAPDH Antibody Catalog Number: ARP40175_P050 Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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