Ehf Antibody - N-terminal region (ARP40136_P050)

Data Sheet
Product Number ARP40136_P050
Product Page
Name Ehf Antibody - N-terminal region (ARP40136_P050)
Protein Size (# AA) 300 amino acids
Molecular Weight 35 kDa
NCBI Gene Id 13661
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Ets homologous factor
Alias Symbols AU019492, 9030625L19Rik
Peptide Sequence Synthetic peptide located within the following region: SCIPFQEFDISGEHLCSMSLQEFTRAAGSAGQLLYSNLQHLKWNGQCSSD
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Ko,M.S., (2000) Development 127 (8), 1737-1749
Description of Target Ehf is a transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. It may act as a repressor for a specific subset of ETS/AP-1-responsive genes, and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. It binds to DNA sequences containing the consensus nucleotide core sequence GGAA and involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter
Protein Interactions Nfkbiz;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-Ehf (ARP40136_P050) antibody
Blocking Peptide For anti-Ehf (ARP40136_P050) antibody is Catalog # AAP40136 (Previous Catalog # AAPP23345)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of mouse Ehf
Uniprot ID Q9NZC4
Protein Name ETS homologous factor

He, J. et al. Profile of Ets gene expression in human breast carcinoma. Cancer Biol. Ther. 6, 76-82 (2007). 17172821

Protein Accession # NP_031940
Purification Affinity Purified
Nucleotide Accession # NM_007914
Tested Species Reactivity Human, Mouse
Gene Symbol Ehf
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 93%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 93%
Image 1
Mouse Prostate
WB Suggested Anti-Ehf Antibody Titration: 0.2-1 ug/ml
Positive Control: Mouse Prostate
Image 2
Human kidney
Human kidney
Image 3

25 ug of the indicated Mouse whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. An isoform containing the peptide sequence is present at ~31 kDa.

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