NOTCH1 Antibody - middle region (ARP39295_P050)

• Product Number: ARP39295_P050
• Name: NOTCH1 Antibody - middle region (ARP39295_P050)
• Protein Size (# AA): 2555 amino acids
• Molecular Weight: 273 kDa
• NCBI Gene Id: 4851
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Notch 1
• Alias Symbols: hN1, AOS5, TAN1, AOVD1
• Peptide Sequence: Synthetic peptide located within the following region: EHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Sahlgren,C., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (17), 6392-6397
• Description of Target: NOTCH1 is a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: USP12; UBC; TGFBR1; WWP1; FBXW7; EGFL7; LATS2; RBPJ; ITCH; NEDD4L; ARRB1; MAML1; Su(H); XIAP; MDM2; CAMK4; NUMB; MCM5; MCM3; SMAD9; LIMK2; IPO5; TNPO1; KPNB1; KIF2A; IFI16; NCKAP1L; HDAC1; HCLS1; HCFC1; GPS1; ERH; DHX15; DHX8; CHD4; CDC27; LRBA; CCT6A; RU
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Specificity: Antibody recognizes 88 kDa for Notch1 intracellular domain.
• Blocking Peptide: For anti-NOTCH1 (ARP39295_P050) antibody is Catalog # AAP39295 (Previous Catalog # AAPP23028)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NOTCH1
• Uniprot ID: P46531
• Protein Name: Neurogenic locus notch homolog protein 1
• Protein Accession #: NP_060087
• Purification: Affinity Purified
• Nucleotide Accession #: NM_017617
• Tested Species Reactivity: Human
• Gene Symbol: NOTCH1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 79%; Dog: 79%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 79%; Rat: 93%; Zebrafish: 93%
• Image 1: Human 293T
  
  WB Suggested Anti-NOTCH1 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: 293T cell lysate
• Image 2: Human U937 Whole Cell
  
  Host: Rabbit
  Target Name: NOTCH1
  Sample Tissue: Human U937 Whole Cell
  Antibody Dilution: 1ug/ml
• Image 3: Human Lung
  
  Rabbit Anti-NOTCH1 Antibody
  Catalog Number: ARP39295_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
  Observed Staining: Plasma membrane
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec