ILF3 Antibody - N-terminal region (ARP38968_P050)

• Product Number: ARP38968_P050
• Name: ILF3 Antibody - N-terminal region (ARP38968_P050)
• Protein Size (# AA): 894 amino acids
• Molecular Weight: 95kDa
• NCBI Gene Id: 3609
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Interleukin enhancer binding factor 3, 90kDa
• Alias Symbols: CBTF, DRBF, MMP4, MPP4, NF90, NFAR, NF110, NF90a, NF90b, NF90c, NFAR2, TCP80, DRBP76, NF110b, NFAR-1, NFAR-2, NFAR90, TCP110, MPP4110, NF90ctv, NFAR110, MPHOSPH4, NF-AT-90
• Peptide Sequence: Synthetic peptide located within the following region: IFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Nie,Y., (2005) Mol. Cell. Biol. 25 (16), 6956-6963
• Description of Target: Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of ILF3.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.
• Protein Interactions: PLSCR1; UBC; FBXW11; RBPMS; HUWE1; MDM2; AURKA; SUMO2; SUMO3; STAU1; IVNS1ABP; LGR4; ERG; RPA3; RPA2; RPA1; EZH2; HNRNPL; SUZ12; RNF2; BMI1; EED; VHL; VEGFA; rev; FBXO6; TARDBP; CSF2; EIF2AK2; ICAM1; IGSF8; SNORD116-22; PABPC1; RNU2-4P; RNU5B-1; RNU5A-1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Specificity: The immunizing peptide used to raise this antibody is 100% homologous to isoform 2 (702aa, 76kDa), 3 (764aa, 83kDa), 4 (698aa, 76kDa) and 5 (690aa, 75kDa), 6 (706aa, 77kDa) and 7 (898aa, 96kDa) of human ILF3.
• Blocking Peptide: For anti-ILF3 (ARP38968_P050) antibody is Catalog # AAP38968 (Previous Catalog # AAPY00776)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ILF3
• Uniprot ID: Q12906-2
• Protein Name: Interleukin enhancer-binding factor 3
• Sample Type Confirmation:

  ILF3 is strongly supported by BioGPS gene expression data to be expressed in K562

• Protein Accession #: NP_004507
• Purification: Affinity Purified
• Nucleotide Accession #: NM_004516
• Tested Species Reactivity: Human, Rat
• Gene Symbol: ILF3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
• Image 1: Human Lung
  
  Human Lung
• Image 2: rat aorta
  
  WB Suggested Anti-ILF3 Antibody
  Titration: 1 ug/ml
  Positive Control: Rat tissue
• Image 3: Human Pineal Tissue
  
  Rabbit Anti-ILF3 Antibody
  Catalog Number: ARP38968_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
  Observed Staining: Cytoplasmic and nuclear in pinealocytes
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1/600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 4: Human Stomach Tumor
  
  Host: Rabbit
  Target Name: ILF3
  Sample Tissue: Human Stomach Tumor
  Antibody Dilution: 1.0ug/ml