Product Number |
ARP38820_P050 |
Product Page |
www.avivasysbio.com/ctcf-antibody-n-terminal-region-arp38820-p050.html |
Name |
CTCF Antibody - N-terminal region (ARP38820_P050) |
Protein Size (# AA) |
727 amino acids |
Molecular Weight |
83kDa |
NCBI Gene Id |
10664 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
CCCTC-binding factor (zinc finger protein) |
Description |
|
Alias Symbols |
MRD21, FAP108, CFAP108 |
Peptide Sequence |
Synthetic peptide located within the following region: MEGDAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEV |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Defossez,P.A., (2005) J. Biol. Chem. 280 (52), 43017-43023 |
Description of Target |
CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. |
Protein Interactions |
ZMYM4; UBC; EED; BMI1; KANSL1; CDK19; ZMYM2; CAND1; ZBTB33; TFAP4; SUMO1; SUMO2; SUMO3; SIRT7; TAF3; SMC3; POLR2A; SUZ12; Ctcf; HIST2H2AC; TOP2A; TAF1; NPM1; LMNA; KPNA4; KPNA2; H2AFZ; PARP1; TRB; RXRA; SMAD3; SIN3A; SMC1A; RAD21; YY1; THRB; SMAD4; HMGB1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-CTCF (ARP38820_P050) antibody |
Blocking Peptide |
For anti-CTCF (ARP38820_P050) antibody is Catalog # AAP38820 (Previous Catalog # AAPP21028) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human CTCF |
Uniprot ID |
P49711 |
Protein Name |
Transcriptional repressor CTCF |
Publications |
Folding Keratin Gene Clusters during Skin Regional Specification. Dev Cell. 53, 561-576.e9 (2020) 32516596 |
Protein Accession # |
NP_006556 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_006565 |
Tested Species Reactivity |
Human |
Gene Symbol |
CTCF |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
CHIP, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
Image 1 | Transfected 293T
| WB Suggested Anti-CTCF Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T |
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Image 2 | HCT116
| Chromatin Immunoprecipitation (ChIP) Using CTCF antibody - N-terminal region (ARP38820_P050) and HCT116 Cells |
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