CTCF Antibody - N-terminal region (ARP38820_P050)

Data Sheet
 
Product Number ARP38820_P050
Product Page www.avivasysbio.com/ctcf-antibody-n-terminal-region-arp38820-p050.html
Name CTCF Antibody - N-terminal region (ARP38820_P050)
Protein Size (# AA) 727 amino acids
Molecular Weight 83kDa
NCBI Gene Id 10664
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name CCCTC-binding factor (zinc finger protein)
Description
Alias Symbols MRD21, FAP108, CFAP108
Peptide Sequence Synthetic peptide located within the following region: MEGDAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEV
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Defossez,P.A., (2005) J. Biol. Chem. 280 (52), 43017-43023
Description of Target CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.
Protein Interactions ZMYM4; UBC; EED; BMI1; KANSL1; CDK19; ZMYM2; CAND1; ZBTB33; TFAP4; SUMO1; SUMO2; SUMO3; SIRT7; TAF3; SMC3; POLR2A; SUZ12; Ctcf; HIST2H2AC; TOP2A; TAF1; NPM1; LMNA; KPNA4; KPNA2; H2AFZ; PARP1; TRB; RXRA; SMAD3; SIN3A; SMC1A; RAD21; YY1; THRB; SMAD4; HMGB1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-CTCF (ARP38820_P050) antibody
Blocking Peptide For anti-CTCF (ARP38820_P050) antibody is Catalog # AAP38820 (Previous Catalog # AAPP21028)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CTCF
Uniprot ID P49711
Protein Name Transcriptional repressor CTCF
Publications

Folding Keratin Gene Clusters during Skin Regional Specification. Dev Cell. 53, 561-576.e9 (2020) 32516596

Protein Accession # NP_006556
Purification Affinity Purified
Nucleotide Accession # NM_006565
Tested Species Reactivity Human
Gene Symbol CTCF
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application CHIP, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Image 1
Transfected 293T
WB Suggested Anti-CTCF Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T
Image 2
HCT116
Chromatin Immunoprecipitation (ChIP) Using CTCF antibody - N-terminal region (ARP38820_P050) and HCT116 Cells
 

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