NR1H3 Antibody - C-terminal region (ARP38670_P050)

• Product Number: ARP38670_P050
• Name: NR1H3 Antibody - C-terminal region (ARP38670_P050)
• Protein Size (# AA): 447 amino acids
• Molecular Weight: 50 kDa
• NCBI Gene Id: 10062
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Nuclear receptor subfamily 1, group H, member 3
• Alias Symbols: LXRA, LXR-a, RLD-1
• Peptide Sequence: Synthetic peptide located within the following region: IHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWD
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Torra,I.P., (2008) Mol. Cell. Biol. 28 (8), 2626-2636
• Description of Target: Interaction of NR1H3 with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. NR1H3 are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. NR1H3 Plays an important role in the regulation of cholesterol homeostasis.
• Protein Interactions: RXRG; RXRB; MDFI; SUV39H1; KDM1A; BARD1; MSTO1; HSP90AA1; PIAS1; APP; NR3C1; MYC; ZXDC; TMEM161A; ING3; NCOA6; NCOR1; KAT2B; EIF3I; NRIP1; NCOA3; SRC; RXRA; FOXO3; ACTN1; SS18L1; LZTR1; EDF1; RARA; NR0B2; SYT1; PPARA; SIRT1; UBC; CEP350;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-NR1H3 (ARP38670_P050) antibody is Catalog # AAP38670 (Previous Catalog # AAPP20859)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NR1H3
• Uniprot ID: Q13133
• Protein Name: Oxysterols receptor LXR-alpha
• Protein Accession #: NP_005684
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005693
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: NR1H3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1:
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The peptide is also present in a 28 kDa isoform.