TAF6 Antibody - C-terminal region (ARP38653_P050)

• Product Number: ARP38653_P050
• Name: TAF6 Antibody - C-terminal region (ARP38653_P050)
• Protein Size (# AA): 677 amino acids
• Molecular Weight: 73kDa
• Subunit: 6
• NCBI Gene Id: 6878
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa
• Alias Symbols: ALYUS, TAF2E, TAFII70, TAFII80, TAFII85, MGC:8964, TAFII-70, TAFII-80, TAF(II)70, TAF(II)80
• Peptide Sequence: Synthetic peptide located within the following region: PSVQPIVKLVSTATTAPPSTAPSGPGSVQKYIVVSLPPTGEGKGGPTSHP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Beausoleil,S.A., (2006) Nat. Biotechnol. 24 (10), 1285-1292
• Description of Target: TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1,the largest subunit of TFIID. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
• Protein Interactions: SOX5; HNF4A; MED26; TRRAP; HIST3H3; UBC; TBP; AHR; APP; TAF1L; TAF3; TAF9B; TAF10; TAF9; TAF7; TAF5; TAF4; TAF2; TAF1; KMT2A; USF1; ERCC1; TAF8; KAT2A; SETD7; NR1D2; NCOR1; NFYC; NFYB; NFYA; MBD3; SIN3A; ALL1; SAP30; SMARCC2; SMARCC1; SMARCB1; SMARCA2; RB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TAF6 (ARP38653_P050) antibody is Catalog # AAP38653 (Previous Catalog # AAPP20843)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TAF6
• Uniprot ID: P49848
• Protein Name: Transcription initiation factor TFIID subunit 6
• Protein Accession #: NP_005632
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005641
• Tested Species Reactivity: Human
• Gene Symbol: TAF6
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB, CHIP
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%
• Image 1: Transfected 293T
  
  WB Suggested Anti-TAF6 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: Transfected 293T
• Image 2: HCT116
  
  Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.